Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2020, Vol. 31 ›› Issue (7): 67 doi: 10.1007/s41365-020-00777-8


Study on the multiple characteristics of M3 generation of pea mutants obtained by neutron irradiation

Da-Peng Xu1,3 • Ze-En Yao1,3 • Jian-Bin Pan2 • Hu-Yuan Feng2 • Zhi-Qi Guo1,3 • Xiao-Long Lu1,3   

  1. 1School of Nuclear Science and Technology, Lanzhou University, Lanzhou 730000, China
    2School of Life Sciences, Lanzhou University, Lanzhou 730000, China
    3Engineering Research Center for Neutron Application Technology, Ministry of Education, Lanzhou University, Lanzhou 730000, China
  • Received:2020-03-19 Revised:2020-05-11 Accepted:2020-05-13
  • Contact: Da-Peng Xu E-mail:
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Da-Peng Xu, Ze-En Yao, Jian-Bin Pan, Hu-Yuan Feng, Zhi-Qi Guo, Xiao-Long Lu. Study on the multiple characteristics of M3 generation of pea mutants obtained by neutron irradiation.Nuclear Science and Techniques, 2020, 31(7): 67     doi: 10.1007/s41365-020-00777-8

Abstract: Irradiation breeding is an important technique in the effort to solve food shortages and improve the quality of agricultural products. In this study, a field test was implemented on the M3 generation of two mutant pea plants gained from previous neutron radiation of pea seeds. The relationship between agronomic characteristics and yields of the mutants was investigated. Moreover, differences in physiological and biochemical properties and seed nutrients were analyzed. The results demonstrated that the plant height, effective pods per plant, and yield per plant of mutant Leaf-M1 were 45.0%, 43.2%, and 50.9% higher than those of the control group. Further analysis attributed the increase in yield per plant to the increased branching number. The yield per plant of mutant Leaf-M2 was 7.8% higher than that of the control group, which could be related with the increased chlorophyll content in the leaves. There was a significant difference between the two mutants in the increase of yield per plant owing to morphological variation between the two mutants. There were significant differences in SOD activity and MDA content between the two mutants and the control, indicating that the physiological regulation of the two mutants also changed. In addition, the iron element content of seeds of the two mutants were about 10.9% lower than in the seeds of the control group, a significant difference. These findings indicate that the mutants Leaf-M1 and Leaf-M2 have breeding value and material value for molecular biological studies.

Key words: Neutron radiation, Irradiation breeding, Pea mutant, Agronomic characteristic