Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2018 Impact factor 0.961

Nuclear Science and Techniques ›› 2018, Vol. 29 ›› Issue (6): 77 doi: 10.1007/s41365-018-0421-1

• NUCLEAR CHEMISTRY,RADIOCHEMISTRY,RADIOPHARMACEUTICALS AND NUCLEAR MEDICINE •     Next Articles

Assessment of mouse BMMNC DNA damage with a two-tailed comet assay after X-ray and carbon ion total body irradiation

Fang Liu 1,2 • Zhuan-Zi Wang 1 • Wen-Jian Li 1 • Wei Wei 1 • Bing-Rong Dang 1   

  1. 1 Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China
    2 University of Chinese Academy of Sciences, Beijing 100049, China
  • Contact: Zhuan-Zi Wang E-mail:wangzz@impcas.ac.cn
  • Supported by:

    This work was supported by the National Natural Science Foundation of China (No. 11575259).

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Fang Liu, Zhuan-Zi Wang, Wen-Jian Li, Wei Wei, Bing-Rong Dang. Assessment of mouse BMMNC DNA damage with a two-tailed comet assay after X-ray and carbon ion total body irradiation.Nuclear Science and Techniques, 2018, 29(6): 77     doi: 10.1007/s41365-018-0421-1

Abstract:

The two-tailed comet assay (2T-comet assay) is a method for simultaneously evaluating DNA single-strand breaks (SSBs) and double-strand breaks (DSBs). In the present study, the endonuclease DNase I and hydrogen peroxide were used to induce DSBs and SSBs in bone marrow mononuclear cells (BMMNCs) from mice, and the damaged DNAs were assessed with a 2T-comet assay. The results demonstrated that this method can detect and discriminate between BMMNC DNA SSBs and DSBs simultaneously. Using this method, we studied DNA damage in BMMNCs from female BALB/c mice after total body irradiation with X-rays and carbon ions. The results indicated that these two types of radiation induced serious DNA damage in BMMNCs in a dose-dependent manner. The DNA damage induced by carbon ions was more severe than that induced by X-rays at the same dose, and a high dose of carbon ion radiation was more likely to cause death in mice. The DSBs and SSBs induced by X-rays were the highest on the 3rd day post-IR. For carbon ion radiation, DSBs were the most serious on the 3rd day, while SSBs were more obvious on the 3rd day and 13th day post-IR. The ratio of DSBs/SSBs was clearly related to the different types of radiation.

Key words: Two-tailed comet assay, carbon ion irradiation, X-ray irradiation, Bone marrow mononuclear cells, DNA damage