Nuclear Science and Techniques

《核技术》(英文版) ISSN 1001-8042 CN 31-1559/TL     2019 Impact factor 1.556

Nuclear Science and Techniques ›› 2018, Vol. 29 ›› Issue (5): 67 doi: 10.1007/s41365-018-0407-z

• NUCLEAR CHEMISTRY,RADIOCHEMISTRY,RADIOPHARMACEUTICALS AND NUCLEAR MEDICINE • Previous Articles     Next Articles

Establishment and validation of a method for cell irradiation in 96-well and 6-well plates using a linear accelerator

Xiao-Qing Dong 1 • Qing Lin 1 • Jie Hu 1 • Liang Huang 2 • Kun Yue 3 • Lu Wang 1 • Jia-Fei Zhang 1 • Mei-Ling Lu 4   

  1. 1 Department of Radiation Oncology, Tenth People’s Hospital Affiliated to Tongji University, Shanghai 200072, China
    2 Medical Equipment Department, Tenth People’s Hospital Affiliated to Tongji University, Shanghai 200072, China
    3 Department of Radiation Oncology, (North) NO.9 People’s Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 201999, China
    4 Central Lab, Tenth People’s Hospital Affiliated to Tongji University, Shanghai 200072, China
  • Contact: Qing Lin E-mail:linqing.linda@163.com
  • Supported by:

    This work was supported by the Hospital Personnel Climbing Plan of the Tenth People’s Hospital Affiliated to Tongji University.

PDF ShareIt Export Citation
Xiao-Qing Dong, Qing Lin, Jie Hu, Liang Huang, Kun Yue, Lu Wang, Jia-Fei Zhang, Mei-Ling Lu. Establishment and validation of a method for cell irradiation in 96-well and 6-well plates using a linear accelerator.Nuclear Science and Techniques, 2018, 29(5): 67     doi: 10.1007/s41365-018-0407-z

Abstract:

To establish and validate a method for cell irradiation in 96-well and 6-well plates using a linear accelerator, three irradiation methods (G0B0F40, G0B1.5F40, and G180B1.5F40) were designed to irradiate cell culture plasticware simulated with RW3 slab phantom and polystyrene. The difference between the actual physical measured dose and the preset dose was compared among the three methods under the preparatory conditions of 2, 4, 6, 8, and 10 Gy. MDA-MB-231 cells were analyzed by using a cell proliferation assay and a clonogenic assay to verify the difference between the three cell irradiation methods on cell radiosensitivity. For each preset dose, the difference between the actual measured dose and the preset dose was the lowest for Method G0B1.5F40, the second lowest for Method G180B1.5F40, and the maximum for Method G0B0F40. The ranges of the differences were - 0.28 to 0.02%, - 2.17 to - 1.80%, and - 4.92 to - 4.55%, and 0.31 to - 0.12%, - 3.42 to - 2.86%, and - 7.31 to - 6.92%, respectively, for 96-well and 6-well plates. The cell culture experiments proved that Method G0B1.5F40 was an accurate, effective, simple, and practical irradiation method. The most accurate and effective cell irradiation method should always be used, as it will reduce dose differences and instability factors and provide improved accuracy and comparability for laboratories researching cellular radiosensitivity.

Key words: Linear accelerator, Radiotherapy, Cell irradiation methods, Cell culture plate, Dose