Nuclear Techniques ›› 2019, Vol. 42 ›› Issue (9): 90501-090501.doi: 10.11889/j.0253-3219.2019.hjs.42.090501

• NUCLEAR PHYSICS, INTERDISCIPLINARY RESEARCH • Previous Articles     Next Articles

The effect of graphene oxide nanosheets on the dissociation of amyloid fibers

Peng YAN1,Gengyang HUO1,Xinxing CHU1,Gongjun ZHANG2,Bin LI3,Xingfei ZHOU1()   

  1. 1. School of Physical, Ningbo University, Ningbo 315211, China
    2. Ningbo Institute of Material Technology and Engineering, Chinese Academy of Sciences, Ningbo 315211, China
    3. Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
  • Received:2019-05-29 Revised:2019-07-03 Online:2019-09-10 Published:2019-09-18
  • Contact: Xingfei ZHOU E-mail:zhouxingfei@nbu.edu.cn
  • About author:YAN Peng, male, born in 1991, graduated from Jing Gang Shan University in 2015, master student, focusing on effect of amyloid protein on neurodegenerative diseases
  • Supported by:
    National Natural Science Foundation of China(11474173);Natural Science Foundation of Zhejiang Province(LY18A040003);Ningbo Natural Fund(2018A610220)

Abstract: Background

The formation of amyloid fibers is closely related to the neurodegenerative diseases. Inhibiting the formation of mature amyloid fibers has become an important approach to prevent related diseases.

Purpose

This work aims to prepare an efficient and rapid material to remove and degrade mature insulin fibers for the treatment of type II diabetes mellitus.

Methods

In this study, the effect of graphene oxide nanosheets (GOs) with an average thickness of 1 nm on mature insulin fibers was prepared by Hummer method. Both the ThT fluorescence and Micro Fourier Transform Infrared Spectroscopy (Micro-FTIR) were employed to measure the outputs.

Results

GOs can effectively dissociate mature insulin fibers into short fibers and remove them. The dissociation effect is significantly enhanced with the increase of GOs concentration.

Conclusions

Combining the results of ThT fluorescence and Micro-FTIR, speculate that GOs changes the second structures of the mature insulin fibers by extracting the protein molecules, transforming it from the original beta fold to an alpha spiral structure.

Key words: Graphene oxide nanosheets, Insulin fibers, Degradation