Nuclear Techniques ›› 2014, Vol. 37 ›› Issue (07): 70501-070501.doi: 10.11889/j.0253-3219.2014.hjs.37.070501

• NUCLEAR PHYSICS, INTERDISCIPLINARY RESEARCH • Previous Articles     Next Articles

Research on pre-staining gel electrophoresis

ZHONG Ruibo LIU Yushuang ZHANG Ping LIU Jingran ZHAO Guofen ZHANG Feng   

  1. (College of Life Science, Inner Mongolia Agricultural University, Huhhot 010018, China)
  • Received:2014-04-15 Revised:2014-04-22 Online:2014-07-10 Published:2014-07-07

Abstract:

Background: Gel electrophoresis is a powerful biochemical separation technique. Most biological molecules are completely transparent in the visible region of light, so it is necessary to use staining to show the results after gel electrophoresis, and the general steps of conventional staining methods are time-consuming. Purpose: We try to develop a novel approach to simplify the gel electrophoresis: Pre-Staining Gel Electrophoresis (PSGE), which can make the gel electrophoresis results monitored in real time. Methods: Pre-stain the protein samples with Coomassie Brilliant Blue (CBB) for 30 min before loading the sample into the gel well. Results and Conclusion: PSGE can be successfully used to analyze the binding efficiency of Bovine Serum Albumin (BSA) and amphiphilic polymer via chemical coupling and physical absorption, and the double PSGE also shows a great potential in bio-analytical chemistry.

Key words: Gel electrophoresis, Pre-staining, Covalent coupling, Physical absorption, Protein, Amphiphilic polymer