Nuclear Techniques ›› 2017, Vol. 40 ›› Issue (8): 80102-080102.doi: 10.11889/j.0253-3219.2017.hjs.40.080102


Electrospinning sample delivery for serial crystallography using synchrotron radiation

CHEN Jianqiao1,2, WANG Qisheng1,2, LI Bing1,2, ZHOU Huan1, CUI Ying1, SUN Bo1, WANG Zhijun1, HE Jianhua1   

  1. 1. Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Zhangjiang Campus, Shanghai 201204, China;
    2. University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2017-04-11 Revised:2017-04-28 Online:2017-08-10 Published:2017-08-11
  • Supported by:
    Supported by Strategic Priority Research Programmes (B) of Chinese Academy of Sciences (No.XDB08030101),Youth Innovation Promotion Association of Chinese Academy of Sciences (No.2015213),National Natural Science Foundation of China (No.U1632126)

Abstract: Background: As a novel approach to solve the protein structure, serial femtosecond crystallography (SFX) has been developed rapidly owing to the advantages of the room-temperature data collection, lower radiation damage and time-resolved study. The first X-ray free-electron laser (XFEL) based SFX experiment has offered a new method to structure determination of protein microcrystals successfully. Purpose:The aim is to introduce SFX into the widely-available third-generation synchrotron radiation (SR). Methods: The electrospinning sample delivery system is a potential method to be applied on SR. The theoretical and experimental researches on electrospinning were studied using BL17U1 beamline in Shanghai synchrotron radiation facility (SSRF). Results: A microjet containing microcrystals was produced from the liquid meniscus and controlled by an electrostatic field. The air ionization problem disturbing electrospinning in SR experiment was solved by providing vacuum condition and coaxial electrospinning technique. Using lysozyme microcrystals as a model sample, the feasibility of electrospinning was demonstrated. Conclusion: It is shown that the serial crystallography on millisecond time scale is suitable for SR, which provides a reference for the future beamline upgrading.

Key words: Serial crystallography, Synchrotron radiation, Electrospinning, Room-temperature data collection, Protein microcrystal

CLC Number: 

  • TL99