Nuclear Techniques ›› 2020, Vol. 43 ›› Issue (9): 90501-090501.doi: 10.11889/j.0253-3219.2020.hjs.43.090501


Application of plasmonic nanoprobes in cell imaging

Wenhan LIU1,2,Xiaodong XIE1,2,Min YIN3,Nan CHEN3()   

  1. 1.Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
    2.University of Chinese Academy of Sciences, Beijing 100049, China
    3.College of Chemistry and Materials Science, Shanghai Normal University, Shanghai 200234, China
  • Received:2020-04-04 Revised:2020-05-19 Online:2020-09-15 Published:2020-09-09
  • Contact: Nan CHEN
  • About author:LIU Wenhan, female, born in 1995, graduated from Chongqing University in 2016, doctoral student, focusing on nanoprobes for cell imaging
  • Supported by:
    National Natural Science Foundation of China(31771102)


Noble metal nanomaterials possess localized surface plasmon resonance (LSPR) effect. They are widely utilized in the fields of nanophotonics, chemical sensing, targeted radiation therapy, and bioimaging due to their unique physical, chemical, and optical properties. By adjusting the composition, morphology and size of the plasmonic nanoparticles, probes with distinct light scattering properties can be prepared and then monitored by dark-field microscopy (DFM). Compared with the fluorescent probes, plasmonic nanoprobes have better sensitivity and stability, which is suitable for live-cell imaging and real-time tracking of a single probe. Here, the latest development and some examples of plasmonic nanoprobes for dark-field imaging are introduced. Application in the observation of cell membrane receptors, in situ detection of intracellular biomolecules, and intracellular tracking are summarized, and perspects of plasmonic nanoprobes in DFM cell imaging are provided.

Key words: Plasmonic nanoprobes, Dark-field microscopy, Cell imaging, Single particle analysis

CLC Number: 

  • O651