Nuclear Techniques ›› 2019, Vol. 42 ›› Issue (6): 60101-060101.doi: 10.11889/j.0253-3219.2019.hjs.42.060101

• SYNCHROTRON RADIATION TECHNOLOGY AND APPLICATIONS •     Next Articles

Chip-based efficient data collection technology from multiple crystals

Bing LI1,2,Qisheng WANG1,3,Minjun LI3,Haojie GUO1,2,Li YU1,2,Jianhua HE1,3()   

  1. 1. Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
    2. University of Chinese Academy of Sciences, Beijing 100049, China
    3. Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China
  • Received:2019-01-24 Revised:2019-04-25 Online:2019-06-10 Published:2019-06-18
  • Contact: Jianhua HE E-mail:hejianhua@sinap.ac.cn
  • About author:LI Bing, female, born in 1990, graduated from University of South China in 2013, doctoral student, focusing on synchrotron technology
  • Supported by:
    国家重点研发计划(No.2017YFA0504901)、中国科学院青年创新促进会资助((No.2015213));Supported by National Key Research and Development Plan of China (No.2017YFA0504901), Youth Innovation Promotion Association of Chinese Academy of Sciences(No.2015213)

Abstract: Background

X-ray crystallography is still the most important means of obtaining atomic resolution structure of biological macromolecules. Obtaining protein crystals of sufficient size is the bottleneck of crystallography. Small crystals are easier to obtain than large crystals, and lattice packing is better. But small crystals are susceptible to radiation damage, and it is difficult to obtain complete structural information from a crystal even when data collection is performed at low temperatures.

Purpose

This paper aims to propose a method for acquiring small crystal data based on chip techniques at a microfocus beam line station of synchrotron radiation light source.

Methods

First of all, the chip was fabricated by laser lithography technology, and crystal was able to be deposited in the groove of the chip. Then, high-throughput data collection for diffraction of multi-crystals on the chip was achieved on the chip, and collected multiple chips data sets were emerged to get structure information of biological macromolecules. Finally, the feasibility of chip-based sample delivery method was verified by using the lysozyme crystal at the microfocus beamline (BL18U1) of SSRF.

Results

The quartz chip has low background scattering and high sample delivery efficiency. The structure of lysozyme with high resolution of 0.139 nm was obtained by chip loading and data acquisition.

Conclusions

The chip-based polycrystalline data acquisition method can obtain complete biomacromolecule structural information without obvious radiation damage by merging multiple data sets.Key words X-ray crystallography, High-throughput sample delivery method, Radiation damage, Chip