Nuclear Techniques ›› 2018, Vol. 41 ›› Issue (11): 110504-110504.doi: 10.11889/j.0253-3219.2018.hjs.41.110504


Regulation of the structure and mechanical properties of biofilm by medium phase

LIU Shima1,2, LYU Min2, LUO Liqiang1, GUO Jingyang1,2, WANG Lihua2, FAN Chunhai2   

  1. 1 College of Sciences, Shanghai University, Shanghai 200444, China;
    2 Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
  • Received:2018-07-16 Revised:2018-09-26 Online:2018-11-10 Published:2018-11-20
  • Supported by:
    Supported by National Key R&D Program of China (No.2016YFA0201200, No.2016YFA0400900), the Open Project of State Key Laboratory of Oral Diseases (No.SKLOD2018OF01), and National Natural Science Foundation of China (No.61571278, No.U1432116)

Abstract: [Background] Bacteria tends to form biofilm to adapt to the unfavorable living environment. Biofilm, a three-dimensional hydrogel-like compound, is composed of bacteria adhering to the substrate and its extracellular polymeric substance (EPS). Biofilm with gel-like structure has been widely used in industrial wastewater treatment, bioengineering and bio-battery.[Purpose] This study aims to simply and effectively regulate biofilm for practical applications.[Methods] Gram-positive bacteria (Bacillus subtilis) and -negative bacteria (Pseudomonas aeruginosa) were selected as model organisms to investigate the effect of different phase media (solid and liquid) on biofilm structure by rheological tests and scanning electron microscopy.[Results] The results showed the gel-like structure of biofilm depended on the medium phase. Compared to the liquid medium, biofilm cultured with solid medium possessed loose, thicker and stronger mechanical strength.[Conclusions] This study provides a simple method for regulating the structure and mechanical properties of biofilm, and also provides insight into the application of biofilm for adsorption of organic pollutants and the design of new biomaterials.

Key words: Medium phase, Biofilm, Microstructure, Mechanical property

CLC Number: 

  • TL99