Nuclear Techniques ›› 2018, Vol. 41 ›› Issue (3): 30301-030301.doi: 10.11889/j.0253-3219.2018.hjs.41.030301

• NUCLEAR CHEMISTRY, RADIOCHEMISTRY, RADIOPHARMACEUTICALS AND NUCLEAR MEDICINE • Previous Articles     Next Articles

Acute apoptosis of mice bone marrow dendritic cells induced by ionizing irradiation

ZHANG Hong1,2, LUO Bingzhi1, XU Fenglan1, HU Xiaoya1, ZHANG Zhe1, LIU Xiaoqin1   

  1. 1. Gansu Wuwei Tumor Hostipal, Wuwei 733000, China;
    2. Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China
  • Received:2017-12-04 Revised:2018-01-17 Online:2018-03-10 Published:2018-03-14
  • Supported by:
    Supported by National Natural Science Foundation of China (No.11605260)

Abstract: [Background] Radiation would induce apoptosis of cells. [Purpose] This study aims to observe the effect of irradiation on bone marrow dendritic cells (BMDCs) in mice. [Methods] The BMDCs were irradiated by X-ray to absorbed doses of 0.5 Gy or 1.0 Gy. The apoptosis and immunity ability of DCs were measured by flow cytometry and CCK-8. Protein expressions were determined by Western blot. The JAK2/STAT3 signal transduction pathway was inhibited by protein inhibitors. [Results] The dysfunction of BMDCs induced by X-ray irradiation was observed. The BMDCs apoptosis percentage increased, and JAK-2, STAT-3, and cleaved Caspase-3 protein expressions were promoted. [Conclusion] The up-regulation of JAK2, STAT3 and cleaved Caspase-3 expression induced by X-ray irradiation results in apoptosis and dysfunction of BMDCs, and may represent a new mechanism in radiation-induced immune suppression.

Key words: Dendritic cells, X-ray, Apoptosis, JAK2/STAT3/Caspase-3 signal pathway

CLC Number: 

  • R594.8