Nuclear Techniques ›› 2017, Vol. 40 ›› Issue (6): 60103-060103.doi: 10.11889/j.0253-3219.2017.hjs.40.060103


Catalytic polymerization-based X-ray imaging tags

ZHU Ying, KONG Huating, ZHANG Jichao, WANG Lihua, HU Jun, FAN Chunhai   

  1. Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Jiading Campus, Shanghai 201800, China
  • Received:2017-04-13 Revised:2017-04-21 Online:2017-06-10 Published:2017-06-06
  • About author:10.11889/j.0253-3219.2017.hjs.40.060103
  • Supported by:
    Supported by Open Large Infrastructure Research of Chinese Academy of Sciences, National Key Research and Development Program (No.2016YFA0400902), National Natural Science Foundation of China (No.11675251, No.21390414), the Youth Innovation Promotion Association of Chinese Academy of Sciences (No.2016236), the Shanghai Sailing Program (No.17YF1423600)

Abstract: Background:With short wavelengths, long penetration depth and excellent energy resolution of X-rays, synchrotron-based X-ray microscopy (XRM) has demonstrated its great potential for nanoscale cellular imaging. However, despite of the high-quality sub-cellular morphological details provided by XRM, it lacks appropriate molecular probes capable of localizing cellular targets.Purpose: This study aims to perform the preparation of X-ray imaging tags. Methods:In vitro chemical catalytic reaction was introduced to get the X-ray imaging tags.Results: By exploiting the excellent energy resolution of X-rays, synchrotron-based X-ray imaging tags were prepared with the imaging resolution of 30 nm when observed by soft X-ray microscopy at Shanghai synchrotron radiation facility (SSRF). Conclusion: The results indicate high potential to design X-ray sensitive molecular probes for cell imaging.

Key words: 3,3'-diaminobenzidine (DAB), Catalysis, Polymer, Synchrotron-based X-ray, Imaging tag

CLC Number: 

  • TL99