Nuclear Techniques ›› 2017, Vol. 40 ›› Issue (4): 40301-040301.doi: 10.11889/j.0253-3219.2017.hjs.40.040301

• NUCLEAR CHEMISTRY, RADIOCHEMISTRY, RADIOPHARMACEUTICALS AND NUCLEAR MEDICINE • Previous Articles     Next Articles

Protective effect of extracted theabrownines from Ya'an Tibetan tea on radiation damage in mice caused by 60Co γ-ray

XU Jingyi1,2,3, LI Xianglong1,2, LI Jie1,2, WU Jiale1,2, TANG Lei1,2, GUO Chengyi4, HE Chunlei1,2,3   

  1. 1 Horticulture College of Sichuan Agricultural University, Chengdu 611830, China;
    2 Laboratory of Tea Science and Engineering, Chengdu 611830, China;
    3 Research Center of Tibetan Tea Engineering Technology, Ya'an 625014, China;
    4 Yi-xing Tibetan Tea Co., Ltd., Ya'an 625014, China
  • Received:2016-11-15 Revised:2017-01-11 Online:2017-04-10 Published:2017-04-07
  • Supported by:

    Supported by Municipal School Cooperation Projects of Ya'an City, Research on Healthy Function of Ya'an Tibetan Tea and Demonstration and Extension of Its Application (No.2016/09-2018/09), and Youth Fund Project of Department of Education of Sichuan Province, Study on the Anti-Radiation Effect of Sichuan Tea (No.12ZA125)

Abstract:

Background: Ya'an Tibetan tea is one of the earliest dark teas in China. It has been drunk for more than a thousand years in the border, and has become a life tea and the main prescription for disease prevention of the ethnic minority. Purpose: The aim is to investigate the protective effect of the theabrownines (TB) of Ya'an Tibetan tea on the damage of 60Co γ-ray radiation by establishing injury mice model caused by 60Co γ radiation. Methods: 48 male SPF mice were randomly divided into normal control group, radiation model group, and positive control group, low, medium and high-dose groups of the TB of Ya'an Tibetan tea. The mice were continuously fed for 15 d. On the 6th day of intragastric administration, all of the mice except for the normal group were subjected to 60Co γ-ray irradiation with a dose of 5 Gy. On the 16th day after weighing, the mice were taken for blood from eyeballs and anatomy. The related indicators of the number of peripheral blood cells, deoxyribonucleic acid (DNA) content of bone marrow, chest spleen index, and total antioxidant capacity of liver tissues were determined. Results: Compared with radiation control group, the TB of Ya'an Tibetan tea could significantly improve the number of white blood cells, platelets and lymphocytes in the blood of radiation-injured mice, and significantly enhance cell activity of blood superoxide dismutase and catalase (p<0.01), and could significantly increase total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD) activity of liver tissue of the mice (p<0.01) and bone marrow DNA content of the femur. At the same time, the content of malondialdehyde (MDA) in liver was significantly decreased (p<0.01). TB could significantly relieve the atrophy of thymus and spleen gland of the immune organs of the mice, and the effect increases with the increase of TB concentration. The effect of high dose group is comparable to or better than the effect of positive drug. Conclusion: The TB of Ya'an Tibetan tea has a good protective effect on the antioxidant system and hematopoietic system of the mice irradiated with 60Co γ-ray, where high-dose group has the most obvious effect.

Key words: TB, Ya'an Tibetan tea, 60Co &gamma, radiation, Antioxidant system, Hematopoietic system, Protection

CLC Number: 

  • TS272