Nuclear Techniques ›› 2016, Vol. 39 ›› Issue (1): 10102-010102.doi: 10.11889/j.0253-3219.2016.hjs.39.010102

• SYNCHROTRON RADIATION TECHNOLOGY AND APPLICATIONS • Previous Articles     Next Articles

An automatic solution-sample-changing peristaltic device at biological small angle X-ray scattering beamline

HONG Chunxia1, ZHOU Ping1, LI Yiwen1,2, ZENG Jianrong1, BIAN Fenggang1, WANG Jie1   

  1. 1 Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Zhangjiang Campus, Shanghai 201204, China;
    2 University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2015-10-26 Revised:2015-12-29 Online:2016-01-10 Published:2016-01-14
  • Supported by:

    Supported by National Key Basic Research Development Program (973 Program) (No.2011CB911104), National Natural Science Foundation of China(No.11305242)

Abstract:

Background: Protein structure in solution can be studied by small-angle X-ray scattering techniques at synchrotron radiation facilities. However, the structure of protein is prone to vary as the radiation damage induced by the intense X-ray beam during the experiments. Purpose: This study aims to develop an automatic solution-sample- changing peristaltic device for biological small angle X-ray scattering experiment. Methods: The injection pump PSD/4 of Hamilton is applied to eliminate the radiation damage of protein by minimizing the X-ray irradiation time of unit volume. In addition, automatic sample-changing, retrieving and cleaning was realized by the coordinated control of PSD/4, the alignment control stage and the sample/buffer control stage. Thus the experiment efficiency is improved. Results: The scattering curves and gyration radius of lysozyme under stationary collection mode and peristaltic collection mode were measured by experiments carried out at biological small angle X-ray scattering beamline of Shanghai Synchrotron Radiation Facility (SSRF). And results show that this device can prevent radiation damage of sample efficiently. Conclusion: The designed functionalities have been achieved as expected.

Key words: EPICS (Experimental Physics and Industrial Control System), BioSAXS, CSS (Control System Studio), Radiation damage

CLC Number: 

  • TP273+.5