Journal of Radiation Research and Radiation Processing ›› 2019, Vol. 37 ›› Issue (1): 10301-010301.doi: 10.11889/j.1000-3426.2019.rrj.37.010301

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Ionizing radiation induces cognitive dysfunction by activating hippocampal astrocytes in mice

WANG Zhen1(),LUO Shishi1,NIU Lei1,NAI Aitao2,XU Yang3,LIU Zhenghai1,HE Jie4,HE Shuya5,ZHONG Xiaolin6,WAN Wei1(),CAO Wenyu1()   

  1. 1. Clinical Anatomy & Reproductive Medicine Application Institute, Medical College, University of South China, Hengyang 421001, China
    2. Department of Radiation Oncology, The First Affiliated Hospital of University of South China, Hengyang 421001, China
    3. Department of Physiology, Medical College, University of South China, Hengyang 421001, China
    4. Department of Pathology, Medical College, University of South China, Hengyang 421001, China
    5. Department of Radiation Medicine, School of Public Health, University of South China, Hengyang 421001, China
    6. Institute of Clinical Medicine, The First Affiliated Hospital of University of South China, Hengyang 421001, China
  • Received:2018-09-03 Revised:2018-11-29 Accepted:2018-11-29 Online:2019-02-20 Published:2019-01-30
  • Contact: Wei WAN,Wenyu CAO E-mail:736887612@qq.com;david-wan@163.com;marksman0@163.com
  • About author:WANG Zhen (female) was born in July 1990, and graduated from University of South China in 2015. Now she is a master candidate in the Clinical Anatomy and Reproductive Medicine Application Institute of University of South China, E-mail:736887612@qq.com|WAN Wei, associate professor, master’s supervisor, E-mail:david-wan@163.com;Ph.D|CAO Wenyu, Lecturer, E-mail:marksman0@163.com
  • Supported by:
    Supported by the Hunan Provincial Natural Science Foundation of China (2018JJ3460, 2018JJ3429, 2018JJ3464), the Hunan Provincial Natural Science Fund Provincial and Municipal Joint Fund Project (2018JJ4034), the Hunan Provincial Education Department Foundation of China (17B230), Planning Project of Hunan Provincial Health and Family Planning Commission Research Projects (C2016042), the Start-up Funds for Ph.D.s in University of South China (2015XQD27, 2015XQD28), Applied Anatomy and Reproductive Medicine Key Laboratory Construction Project in Hengyang (2017KJ182), and Graduate Student Research Innovation Project of University of South China in 2018 (2018KYY256, 2018KYY258, 2018KYZ065, 2018KYZ069)

Abstract:

In order to explore the possible mechanisms underlying cognitive dysfunction induced by ionizing radiation in mice, twenty-four female Kunming mice were randomly divided into a control group and irradiation group (IR). The IR group mice were irradiated with 137Cs γ-rays at an absorbed dose of 4 Gy. After 35 d, the Y-maze test was used to evaluate the cognitive function of the mice. The expression of the astrocyte marker, glial fibrillary acidic protein (GFAP), was detected using immunohistochemical staining. The expression of GFAP protein in the hippocampus was assayed using western blot. The expression of GFAP, interleukin (IL)-1α, IL-1β, and IL-6 mRNA in the hippocampus was detected using reverse transcription polymerase chain reaction. The results showed that compared with the control group, the spontaneous alternation rate of the IR mice was significantly reduced in the Y-maze test (78.40 ± 4.16 vs. 67.53 ± 3.08, p < 0.05); and the number of GFAP-positive cells in the hippocampus was significantly higher (133.4 ± 9.6 vs. 280.3 ± 32.7, p < 0.01); GFAP protein levels (1.000 ± 0.036 vs. 1.300 ± 0.102, p<0.05) and GFAP mRNA expression (1.000 ± 0.0307 vs. 1.203 ± 0.078, p < 0.05) were significantly upregulated; and the expression of inflammatory factors IL-1α, IL-1β, and IL-6 mRNA was upregulated (1.000 ± 0.133 vs 1.677 ± 0.164, p < 0.01; 1.000 ± 0.132 vs. 1.488 ± 0.105, p < 0.05; 1.000 ± 0.218 vs 2.181 ± 0.188, p < 0.01). It is suggested that ionizing radiation induces cognitive dysfunction by activating astrocytes in the hippocampus.

Key words: Ionizing radiation, Hippocampus, Astrocytes, Cognitive dysfunction

CLC Number: 

  • R818.74