Journal of Radiation Research and Radiation Proces ›› 2018, Vol. 36 ›› Issue (1): 10401-010401.doi: 10.11889/j.1000-3436.2018.rrj.36.010401

• RADIATION TECHNOLOGY APPLICATION • Previous Articles     Next Articles

Screening for high teicoplanin-producing strain by mutation combined with resistance to self-produced metabolite

ZHANG Yin, ZHANG Zhulan, QIU Guanrong, YANG Huangjian, WANG Desen, REN Linying, LIAN Yunyang   

  1. Fujian Provincial Key Laboratory of Screening for Novel Microbial Products, Fujian Institute of Microbiology, Fuzhou 350007, China
  • Received:2017-11-01 Revised:2017-12-15 Online:2018-02-20 Published:2018-02-08
  • Supported by:

    Supported by Major Science and Technology Project of Fujian Province of China (2014YZ0004-2), Major Research and Development Platform for Chemical Technology of Fujian Province of China (2014Y2001), Bio-Pharmaceutical Industry Technology Innovation Center of Fuzhou City of China (2016-PT-36), and Special Project of Public Welfare Research Institute of Fujian Province of China (2017R1009-13)

Abstract:

To screen high-yield teicoplanin-producing strain, 60Co γ-ray irradiated spore suspension of the strain Actinoplanes teichomyceticus FIM-16 was spread on regenerating plate containing lethal concentration of teicoplanin. Teicoplanin-resistant mutants were picked and screened by shake-flask fermentation. Strain FIM-16-58 showed two times higher teicoplanin yield than the original strain, and demonstrated higher hereditary stability. There was no obvious effect on the fermentation level of the strain over three generations, and its slope was stable at 4 ℃ for two months. During scale-up experiment in the 1 T fermenter, foam was suppressed effectively by adding 0.1% corn oil, and the yield of teicoplanin reached over 5.0 g/L. Therefore, the high-yield strain laid the foundation of its industrial production and fermentation.

Key words: Actinoplanes teichomyceticus, Resistance mutation screening, Radiation mutagenesis, Teicoplanin

CLC Number: 

  • TL99