Journal of Radiation Research and Radiation Proces ›› 2016, Vol. 34 ›› Issue (6): 60202-60202.doi: 10.11889/j.1000-3436.2016.rrj.34.060202

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles     Next Articles

Expression of FOXM1 and its effects on radiation sensitivity in esophageal squamous cell carcinoma (ESCC)

LIU Langhuan1,2, LUO Aiwu1, CHEN Keliang1, GU Mengmeng2, XIAO Beibei2, ZOU Shitao3, HE Chao3, ZHOU Jundong3, HUANG Bo1   

  1. 1. School of Public Health, University of South China, Hengyang 421001, China;
    2. School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China;
    3. Nanjing Medical University Affiliated Suzhou Hospital, Suzhou 215001, China
  • Received:2016-09-01 Revised:2016-10-30 Online:2016-12-20 Published:2016-12-08
  • Supported by:

    Supported by the National Natural Science Foundation of Hunan Province (2016JJ2115), Doctor stand-up foundation of University of South China (2015XQD31), Health and Family Planning Commission Scientific Research of Hunan Province (C2015-17)

Abstract:

Real-time PCR was used to examine the expression of FOXM1 (Forkhead box M1) in 40 esophageal squamous cell carcinoma (ESCC) tissues, and the adjacent normal tissues. Immunohistochemistry was used to examine the expression of FOXM1 protein in 94 ESCC tissues, and to analyze the relationship between ESCC and clinic pathologic. A small interfering RNA targeting FOXM1 was designed to silence the endogenous FOXM1 expression of ECA-109 and TE-1 cells. The effect of FOXM1 on radiation sensitive in esophageal squamous cell was observed by immunofluorescence stain, and colony formation assay. The results showed that FOXM1 mRNA expression level in esophageal cancer tissues was significantly higher than that of the adjacent tissues (p<0.01), and the protein level expression of it has negative correlation with the survival times; the designed siRNA could dramatically silence FOXM1 expression in ECA-109 cells; γH2AX focus induced by X-rays in FOXM1 silent cells was significantly higher than that in control cells; the clone formation ability of the silent FOXM1 group was significantly lower than that in the control group. The content of FOXM1 is improved in ESCC, and is negatively correlated with the prognosis of patients; the cell radiation sensitive proliferation is significantly improved in the silent FOXM1 ECA-109 cells. In conclusion, FOXM1 has the potential to be the treatment of esophageal squamous carcinoma target.

Key words: Esophageal squamous cell carcinoma, FOXM1 poor prognosis, Radiation sensitivity, DNA damage repair

CLC Number: 

  • TL71