西咪替丁对伽马射线辐照小鼠免疫功能的保护作用

doi:10.11889/j.1000-3436.2018.rrj.36.020203

Journal of Radiation Research and Radiation Proces ›› 2018, Vol. 36 ›› Issue (2): 20203-020203.doi: 10.11889/j.1000-3436.2018.rrj.36.020203

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles Next Articles

Protective effect of cimetidine on immune function of mice irradiated by gamma-rays

HE Ying, ZHANG Junling, ZHAO Yining, JIANG Dingwen, WANG Qingrong, LI Kexian, LUO Qun, SHEN Xianrong

Department of Radiation Protection Medicine, Naval Medical Research Institute, The Second Military Medical University, Shanghai, 200433, China

Received:2017-11-19 Revised:2018-01-20 Online:2018-04-20 Published:2018-04-19
Supported by:
Supported by Ministry Special Drugs Project of National Major Scientific and Technological Special Project for Significant New Drugs Development (2014ZX09J14103-07B)

Abstract

Abstract:

Sixty male mice were divided into six groups:normal control group (0 Gy), model control group (1.0 Gy), positive control group (1.0 Gy+523 tablet), and cimetidine groups (1.0 Gy) at the doses of 33.3, 100, and 300 mg/kg, respectively. The mice were irradiated to absorbed dose of 1.0 Gy with ⁶⁰Co g-rays at 8.33 mGy/min. Cimetidine was administered intragastrically 6 d before irradiation and 5 h after irradiation separately. Twenty-four hours after irradiation, the apoptosis rate of spleen lymphocytes, T lymphocyte subsets, and B lymphocytes in spleen, the content of IL-2, IL-4, IL-6, IL-12, IFN-γ, and TNF-a in serum were detected. Compared with normal control group, the apoptosis rate of spleen lymphocytes increased significantly (p<0.01). The percentage of CD3⁺, CD3⁺CD8⁺ T lymphocytes, CD3⁻CD19⁺ B lymphocytes, and the content of IL-4, IL-12, and TNF-a in serum decreased significantly (p<0.01, p<0.05). Compared with model control group, the apoptosis rate of spleen lymphocytes decreased (p<0.01), while the content of IL-4, IL-6, IL-12, IFN-γ, and TNF-a increased in serum of cimetidine groups (p<0.01, p<0.05). The percentage of CD3⁺, CD3⁺CD4⁺, CD3⁺CD8⁺, and CD3⁻CD19⁺ cells increased in 300 mg/kg cimetidine group (p<0.01, p<0.05). Cimetidine could improve the immune functions of mice irradiated by g-rays to 1.0 Gy.

Key words: Cimetidine, Low-dose-rate radiation, Immune function, Apoptosis

CLC Number:

TL72

HE Ying, ZHANG Junling, ZHAO Yining, JIANG Dingwen, WANG Qingrong, LI Kexian, LUO Qun, SHEN Xianrong. Protective effect of cimetidine on immune function of mice irradiated by gamma-rays[J].Journal of Radiation Research and Radiation Proces, 2018, 36(2): 20203-020203.

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Protective effect of cimetidine on immune function of mice irradiated by gamma-rays

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