Journal of Radiation Research and Radiation Proces ›› 2017, Vol. 35 ›› Issue (1): 10401-010401.doi: 10.11889/j.1000-3436.2017.rrj.35.010401

• RADIATION TECHNOLOGY APPLICATION • Previous Articles     Next Articles

Study on screening and mutation breeding of high-yield Lactobacillus plantarum by heavy ion irradiation

WANG Yuchen1,2, WANG Shuyang1,2, DONG Miaoyin2, XU Fuqiang2, HU Wei2, CHEN Jihong2,3, LI Wenjian2,3, HAN Shunyu1, LIU Jing2   

  1. 1 College of Food Science and Engineering, Gansu Agriculture University, Lanzhou 730070, China;
    2 Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;
    3 Irradiation Mutation Breeding Engineering Laboratory of Gansu Province, Lanzhou 730000, China
  • Received:2016-09-21 Revised:2016-10-30 Online:2017-02-20 Published:2017-02-20
  • Supported by:

    Supported by the National Natural Science Foundation of China (11305225),Science and Technology Service Network Initiative (KFJ-EW-STS-086),and Xizang forage Science and Technology (Y106460XZO)

Abstract:

12C6+ ion beam was used to irradiate strains of Lactobacillus plantarum JTL, and the results showed that the strain could get the highest positive mutation rate and lethality with 34.4% and 80% at the optimal absorbed dose of 300 Gy. The strains which could produce higher lactic acid were screened by selective MRS mediums and fermentation experiments. Finally, three mutations JTL2, JTL3 and JTL5 were obtained through the two screening steps of selecting plate and fermentation, and the contents of lactic acid in this three fermentation broth were greater than the original strain by 41.29% to 49.64%. The pass-generation test indicated that the heredity character of these mutations were stable. It is demonstrated that the lactic acids produced by Lactobacillus plantarum JTL were remarkably improved, and heavy ion beam is an efficient radiation resource for microorganism breeding.

Key words: Lactobacillus plantarum, Heavy ion beam, Irradiation, Mutation breeding

CLC Number: 

  • Q691.5