Journal of Radiation Research and Radiation Proces ›› 2015, Vol. 33 ›› Issue (6): 60202-060202.doi: 10.11889/j.1000-3436.2015.rrj.33.060202

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles     Next Articles

Radioprotective effects of astragaloside IV on the liver cell

HU Yameng  LIU Langhuan  LIU Baochan  XIAO Fangzhu  TANG Yan  HE Shuya  HUANG Bo   

  1. (School of Public Health University of South China, Hengyang 42100, China)
  • Received:2015-09-07 Revised:2015-11-04 Online:2015-12-20 Published:2015-12-07
  • Contact: Ph. D. HUANG Bo, associate professor E-mail:huangbo0930@163.com
  • About author:HU Yameng, Female, was born in June 1989, graduated from preventive medicine specialty of University of South China in 2013, and now is a master candidate in Public Health College of University of South China
  • Supported by:

    Supported by the National Natural Science Foundation of China (81272994), Health and Family Planning Commission Scientific Research of Hunan Province (C2015-17), Education Department Scientific Research of Hunan Province(13C805), and Graduate Student Research Innovation Project of University of South China(2014XCX24)

Abstract:

 L-02 cells were treated with astragaloside IV of different concentrations, and irradiated with γ-rays to establish the radiation damage models. MTT method was used to detect cell viability. Clone formation assay and growth curves were used to observe the effects of astragaloside IV on clone formation and proliferative capability. WST-1 method, microplate test, and DCFH-DA method were used to detect SOD activity, GSH activity, MDA content, and ROS content, respectively. The results showed that astragaloside IV  had no effect on the proliferation of L-02 cells when the concentrations were less than 80 μg/mL. The cell viability decreased with the increasing absorbed doses and time after irradiation. Astragaloside IV could improve the proliferative capability of L-02 cells after irradiation and inhibit the increase of intracellular ROS and MDA contents caused by irradiation, as well as rejuvenate the intracellular SOD and GSH activities to some extent. Therefore, astragaloside IV may reduce the oxidative injury induced by γ-rays by eliminating free radicals within L-02 cells to realize radiation-protective effects.

Key words: Astragaloside IV, Radioprotection, Reactive oxygen species, Liver cell

CLC Number: 

  • TL71