Journal of Radiation Research and Radiation Proces ›› 2015, Vol. 33 ›› Issue (5): 50401-050401.doi: 10.11889/j.1000-3436.2015.rrj.33.050401

• RADIATION TECHNOLOGY APPLICATION • Previous Articles     Next Articles

High-yield glutamic acid strain screened by heavy ion irradiation

MIAO Jianshun1.2.3  CAO Guozhen2.3  ZHANG Miaomiao2.3  ZHANG Wende4  YANG Jianshe1  LI Wenjian2,3  LU Dong2,3   

  1. 1(College of Life Science, Northwest Normal University, Lanzhou 730070, China)
    2 (Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China)
    3 (Key Laboratory of Microbial Resources Exploition and Application, Lanzhou 730000, China)
    4 (Gansu Ronghua Industry Group Co., Ltd., Wuwei 733000, China)
  • Received:2015-05-06 Revised:2015-06-17 Online:2015-10-20 Published:2015-08-31

Abstract:

12C6+ ion beam was used to irradiate glutamic acid-producing strains, and the strains producing higher glutamate were screened by three selective breeding mediums, and then the mutants were fermented by shake flask experiments. Finally the mutants were measured by several fermentation experiments. The results showed that the survival rate of corynebacterium glutamicum changed significantly after irradiation with 80 MeV/u 12C6+ ion beam, and a high-yield strain GM006 was finally obtained through the two screening steps of selecting plate and shaking flask fermentation. The growth rate and glutamic acid yielding of GM006 were remarkably improved. The conversion rate of glucose was 60.55%, which increased by 10.09% compared with the original strain. The highest content of glutamic acid reached 121.10 g/L, and increased by 10.09 % compared with the original strain. This mutant will have broad application prospect as a glutamic acid producing strain, and it is also proved that the heavy ions beam is an efficient radiation resource for microorganism breeding.

Key words: Glutamic acid, Heavy ion beam, Mutation breeding, Irradiation

CLC Number: 

  • Q691.5,TL99