Journal of Radiation Research and Radiation Proces ›› 2015, Vol. 33 ›› Issue (5): 50202-050202.doi: 10.11889/j.1000-3436.2015.rrj.33.050202

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles     Next Articles

Application of carboxyfluorescein-oligonucleotide-carboxytetramethylrhodamine fluorescent probe in dosimetry

MA Hongge  SHI Panying  LIN Wenwen  ZHANG Baoguo   

  1. (School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China)
  • Received:2015-04-29 Revised:2015-05-29 Online:2015-10-20 Published:2015-07-08

Abstract:

To explore the relationship between absorbed dose and fluorescence intensity, FAM-DNA-TAMRA (Carboxyfluorescein-oligonucleotide-carboxytetramethylrhodamine) aqueous solution of different concentrations combined with X-ray irradiation was used to measure the fluorescence intensity. The results showed that the linear relation between fluorescence intensity and absorbed dose was good for different-concentration FAM-DNA-TAMRA aqueous solution containing fluorescent probe in the dose range of 0−20 Gy. The fluorescence had good stability, but the fluorescence background affected the sensitivity of absorbed dose greatly. The results suggest that the FAM-DNA-TAMRA fluorescent probe can be used to measure the absorbed dose of aqueous solution.

Key words: Carboxyfluorescein-oligonucleotide-carboxytetramethylrhodamine fluorescence probe, Fluorescence intensity, Absorbed dose

CLC Number: 

  • TL81,R811