Journal of Radiation Research and Radiation Proces ›› 2015, Vol. 33 ›› Issue (3): 30202-030202.doi: 10.11889/j.1000-3436.2015.rrj.33.030202

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles     Next Articles

Effects of heavy ion irradiation on lung adenocarcinoma A549 cell and its related mechanism

ZHANG Qiuning1,5  YANG Zhen2  CHEN Xinnian3  ZHANG Hong4  LIU Ruifeng1  RAN Juntao1  YANG Xinrui2  WANG Xiaohu1,2,5   

  1. 1(Academy of Medical Sciences, Tumor Hospital of Gansu Province, Lanzhou 730050, China)
    2(School of Life Sciences, Lanzhou University, Lanzhou 730000, China)
    3(Basic Medical College, Lanzhou University, Lanzhou 730000, China)
    4(Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China)
    5(The First Clinical Medical College, Lanzhou University, Lanzhou 730000, China)
  • Received:2015-01-13 Revised:2015-03-06 Online:2015-06-20 Published:2015-04-13


The paper mainly discusses the adhesion, migration and invasion effects and related mechanism of A549 cell irradiated with heavy ion. The logarithmic growth A549 cells were irradiated by different doses carbon heavy ion, and adhesion ability was tested with the MTS method; cell migration and invasion ability were detected with Transwell method; Real time-PCR was performed to detect the expressions of MMP-2 and MMP-9 mRNA. It was found that cell adhesion was significantly enhanced (t=0.014, 0.055, 0.059, p<0.05) after heavy ion (12C6+) rays irradiation and increased with doses, the cell migration and invasion were significantly suppressed with heavy ion (12C6+) irradiation (p<0.05), fluorescence quantitative RT-PCR experiments showed that MMP2 and MMP9 expression were significantly down-regulated (p<0.05) with the increasing of 12C6+ irradiation doses. The results show that heavy ion (12C6+) can improve the adhesive ability of A549 cells and significantly inhibit migration and invasion ability. MMP-2 and MMP-9 expression profiles might be the main molecular mechanism related with the effect.

Key words: Heavy ion, Adhesion, Migration, Invasion, MMPs

CLC Number: 

  • R73,TL99