Journal of Radiation Research and Radiation Proces ›› 2012, Vol. 30 ›› Issue (1): 42-47.doi: 10.11889/j.1000-3436.2012.rrj.30.120108

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Anticancer activity and radiosensitization of CMT-8 in human cervical cancer HeLa cells

SHEN Linling  ZHAO Lin  MA Liang  YANG Jiangtao  JIAO Yang  XU Jiaying  FAN Saijun   

  1. (School of Radiation Medicine and protection, Medical College of Soochow University, Suzhou 215123, China)
  • Received:2011-08-23 Revised:2011-11-01 Online:2012-02-20 Published:2014-09-25

Abstract:

To investigate the effects of chemically modi?ed tetracycline 8 (CMT-8) on cell growth, migration and radio-senstivity in human cervical cancer HeLa cells, MTT assay was used to determine cell viability and radiosensitivity; wound healing and adhesive assays were used to measure cell migration and adhesion; flow cytometry and Western blot assay were performed to analyze cell cycle alteration and protein expression. The results showed that CMT-8 inhibited HeLa cell growth, migration and adhesion in a dose-and time dependent fashion. Furthermore, CMT-8 caused a G0/G1 arrest, in accompany with a dose-dependent down-regulation of Cyclin D and Cyclin E protein expression. Finally, HeLa cells pre-treated with CMT-8 at non-cytotoxic concentrations became more sensitive to subsequent irradiation of X-rays, which was related to a decreased expression of Ku70 and DNA-PKcs protein. Taken together, these findings indicate that CMT-8 is a new anti-cervical cancer compound via inhibiting cell growth and migration as well as mediating radio-sensitivity.

Key words: CMT-8, HeLa cell, X-rays, Radiosensitivity

CLC Number: 

  • R737.33