Journal of Radiation Research and Radiation Proces ›› 2012, Vol. 30 ›› Issue (1): 37-41.doi: 10.11889/j.1000-3436.2012.rrj.30.120107

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The enhancement of radio-sensitivity via autophagy induced by rapamycin with glioma-initiating cells in vivo

ZHAO Jianghu  ZHUANG Wenzhuo  LONG Linmei  LI Feng  LIANG Zhongqin   

  1. (Department of Pharmacology, Medical College, Suzhou University, Suzhou 215123, China)
  • Received:2011-06-10 Revised:2011-10-11 Online:2012-02-20 Published:2014-09-25

Abstract:

To investigate the effect of rapamycin (RPM) on radio-sensitivity of glioma - initiating cells (GICs) in vivo, GICs were inoculated subcutaneously into the NC nude mice. And an intracranial orthotropic model was utilized for evaluation of radio-sensitivity by use of orthotropic injection. Protein levels of LC3 were analyzed with immunoblotting. It was found that tumor inhibition ratio of RPM+IR group was higher than that in control group and IR group. Combination treatment of nude mice bearing intracranial GICs glioma xenografts with rapamycin and IR made a significant increment of animal survival compared with control or IR alone group. Western blot assay showed that the rate of LC3?II /LC3-I increased obviously in combination treatment group among two models. As a conclusion, Rapamycin can enhance the radio-sensitivity of glioma-initiating cells by inducing autophagy in vivo.

Key words: Glioma-initiating cells, Autophagy, Radio-sensitivity, Rapamycin, In vivo test

CLC Number: 

  • R730.264