Journal of Radiation Research and Radiation Proces ›› 2012, Vol. 30 ›› Issue (1): 27-31.doi: 10.11889/j.1000-3436.2012.rrj.30.120105

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Effects of 18F-FDG on the growth of Lewis lung cancer xenografts in C57BL/6 mice

WU Cuiping  QIU Jun  CAI Erpeng  ZHU Mei  SONG Liejing  WANG Mingming   

  1. (Institute of Radiation Medicine, Anhui Medical University, Hefei 230032, China)
  • Received:2011-07-04 Revised:2011-08-19 Online:2012-02-20 Published:2014-09-25

Abstract:

Model of Lewis lung cancer xenograft in C57BL /6 mouse were set up with 18 mice, which were randomly divided into three groups: high-dose 18F-FDG group, low-dose 18F-FDG group and control group. The control was given 0.2 mL saline by intraperitoneal injection 18.5×107 Bq and 9.25×107 Bq 18F-FDG in 0.2 mL saline was given intraperitoneally for one time on the 7th day after Lewis lung cancer cells were inoculated in the high dose 18F-FDG group and low-dose 18F-FDG group, respectively. The effects of 18F-FDG on animals and xenografts were investigated. After 22 days, expression of Bcl-2 and survivin proteins was detected using immunohistochemistry, and apoptotic rate were determined by flow cytometry. The results showed that the inhibition rate and apoptotic rate of xenografts in 18F-FDG-treated groups were higher than that in control group, and the expression of Bcl-2 and Survivin proteins in 18F-FDG-treated groups were less than that in control group (P<0.05). Compared with low-dose 18F-FDG group, there were more significant differences between the high-dose 18F-FDG group and control (P<0.01). It can be suggested that 18F-FDG could effectively inhibit the growth of xenografts. The mechanism may be related to apoptosis caused by the down regulation of bcl-2 and survivin gene expressions in xenografts.

Key words: 18F-FDG, Lewis lung cancer, Apoptosis, bcl-2 gene, Surviving gene

CLC Number: 

  • R817.5