Journal of Radiation Research and Radiation Proces ›› 2012, Vol. 30 ›› Issue (2): 113-119.doi: 10.11889/j.1000-3436.2012.rrj.30.120210

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The effect of inhibition HSP90 on radio-sensitization of hypoxic cancer cells

WANG Huiyu  HONG Chengjiao  ZHANG Baoguo   

  1. (School of Radiation Medicine and Protection of Soochow University, The Key Laboratory
    of Radiation Medicine and Protection of Jiangsu Province, Suzhou 215123,China)
  • Received:2011-10-31 Revised:2011-12-28 Online:2012-04-20 Published:2014-09-25

Abstract:

To investigate the radio-sensitization impact of 17-DAMG on hypoxic human Hela cells, CoCl2 with different concentration was used to treat the cells for 24 h, then the cells were exposed to 17-DAMG of different density for 16 h and irradiated with different doses of 2, 4, 6, 8 Gy γ-rays. The HIF-1α protein level was determined by Western blot analysis, the radiation-sensitization was detected by cloned assay and the Laser Confocal Scanning Microscope was used to test cell apoptosis. The results showed that the HIF-1α protein was over-expressed when the concentration of CoCl2 was set at 200 μmol/L, the cell survival rate was significant decreased (p<0.01) when Hela cells were cultured in different concentration of 17-DAMG and exposed to different doses irradiation; After 17-DAMG pretreatment HIF-1α protein expression was inhibited significantly and the apoptosis rate of hypoxic Hela cells in early and late stages significant increased with the increment of drug concentration (p<0.01). The results indicate that 17-DAMG pretreatment can improve the radio-sensitization of hypoxic Hela cells through the cell apoptosis as HIF-1α protein expression can be inhibited by17-DAMG.

Key words: Cobalt chloride, 17-DAMG, Hypoxia-inducible factor-1, Apoptosis, Radiosensitization

CLC Number: 

  • R811,R811.5