Journal of Radiation Research and Radiation Proces ›› 2012, Vol. 30 ›› Issue (6): 365-369.doi: 10.11889/j.1000-3436.2012.rrj.30.120609

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Study on the biocompatibility of silk fibroin irradiated with different absorbed doses of γ-rays

JIN Huanyu1,2  DONG Chaofei3  LIU Xing1  LIU Yu2,3  WANG Dapeng1  LIU Jian1  JIANG Bingcheng1  YIN Hua2,3  AN Yan1,2   

  1. 1 (School of Public Health, Medical College of Soochow University, Suzhou 215123, China)
    2 (National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, China )
    3 (College of Textile and Clothing Engineering, Soochow University, Suzhou 215006, China)
  • Received:2012-05-29 Revised:2012-07-02 Online:2012-12-20 Published:2014-09-09

Abstract:

To investigate the effects of γ-irradiation on the biocompatibility of silk fibroin, the silk fibroin film was irradiated with different absorbed doses of 0, 25, 50, 100 and 200 kGy, respectively. The dermal cells were used as experimental cell model and cultured on the irradiated films. The molecular structure of silk fibroin was analyzed by FT-IR (Fourier Transform infrared spectroscopy). The graded toxicity and hemolysis had been tested. The results showed that there was no obvious change with the molecular structure of silk fibroin in the range of absorbed dose, meanwhile there was no significant effects on the growth and proliferation activity of dermal cells. And there was also no obvious toxicity to the cells and the silk fibroin film had the good hemo-compatibility. In conclusion, in the range of 0-200 kGy, the γ-irradiation has no significant effects on the structure and biocompatibility of silk fibroin.

Key words: Silk fibroin, γ-irradiation, Molecular structure, Biocompatibility

CLC Number: 

  • R114,R33,TQ316