Journal of Radiation Research and Radiation Proces ›› 2012, Vol. 30 ›› Issue (6): 348-352.doi: 10.11889/j.1000-3436.2012.rrj.30.120606

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Study on the apoptosis effect of MgSO4 on the radiation-induced neural stem cell of rats injury

LIU Ping1  TU Yu2   

  1. 1(Subei People's Hospital of Jiangsu province, Yangzhou 225001, China)
    2(Department of Medical Radioprotection, School of Radiation Medicine and
    Public Health, Suzhou University, Suzhou 215123, China)
  • Received:2012-04-06 Revised:2012-05-16 Online:2012-12-20 Published:2014-09-09

Abstract:

To explore the neuroprotective effect of magnesium sulfate on the apoptosis of neural stem cells induced by radiation, neural stem cells were obtained from newborn sprague-dawley rats cerebral hemisphere, and the identification of neural stem cell was done by immunofluorescence after culturing for 24 h. Neural stem cells were cultured by different concentrations of magnesium sulfate and the right concentration of magnesium sulfate was chosen to be 0.00125 mg/mL. In the experiment the neural stem cells were randomly divided into 3 groups: blank control group, experimental control group (radiation) and experimental group (radiation and treated with magnesium sulfate). The whole neural stem cells of experimental control group and experimental group were irradiated by a dose of 2 Gy or 4 Gy with γ-rays. The apoptosis were detected at different time after irradiation in the range of 24 h, 48 h, and 72 h, respectively. The CCK-8 and the morphology of neural stem cells were observed by TEM. Compared with blank control group, the apoptosis rate of experimental control group significantly increased (t(2Gy、72h)=30.60, t(4Gy、72h)=31.85, p<0.05). Compared with control group, the apoptosis rate of experimental group significantly decreased(t(2Gy、72h)=9.08,t(4Gy,72h)p<0.05). Accordingly, magnesium sulfate can decrease apoptosis rate and reduce the injury of neural stem cells in the early stage after irradiation.

Key words: Neural stem cell, Magnesium sulfate, Ionizing radiation

CLC Number: 

  • R811.5,R329.2,R811.74