Journal of Radiation Research and Radiation Proces ›› 2014, Vol. 32 ›› Issue (5): 6-050205.doi: 10.11889/j.1000-3436.2014.rrj.32.050205

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Screening of biomarker for radiation injury using microassay

WANG Zhidong1  SHEN Liping1  LIN Zhongwu2  ZHANG Xueqing1  LI Xiaoxin1  CHEN Ying1   

  1. 1(Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China)
    2(Ministry of Science and Technolog, Academy of Military Medical Sciences, Beijing 100850, China)
  • Received:2014-05-23 Revised:2014-06-20 Online:2014-10-20 Published:2014-09-04

Abstract:

To screen radiation-sensitive genes with uniform background levels in human peripheral blood, which can be used as biomarkers for radiation injury, peripheral blood samples from three healthy adults were exposed to gamma-rays with dose of 0, 0.75, 2 and 6 Gy. The total RNA was extracted from the whole blood and differential gene expression profiling was analyzed using microarray at 2 h after irradiation. The results showed that 15 differential expressive genes with a relatively uniform background were found: ISG20L1, TRAF4 and TRIAP1 were up-regulated over 2-fold at 2 h after 0.75, 2 and 6 Gy irradiation; LOC345537, POLH, SPIB and TNFSF9 were up-regulated over 2-fold, and TCL1A was down-regulated over 2-fold at 2 h after 2 and 6 Gy irradiation; C12orf5, DDB2, GADD45A, MADCAM1 and RPS27L were up-regulated over 2-fold at 2 h after 6 Gy irradiation.

Key words: Ionizing radiation, Microassay, Radiation-sensitive genes, Biomarkers

CLC Number: 

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