Journal of Radiation Research and Radiation Processing ›› 2019, Vol. 37 ›› Issue (4): 5-040304-7.doi: 10.11889/j.1000-3436.2019.rrj.37.040304

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles     Next Articles

Bioinformatic analysis of ionizing radiation on CAL-27 cells

HU Songling1,2(),HU Qingling4,WANG Weiping2,HE Xiangyi2,DANG Bingrong3,SHAO Chunlin1()   

  1. 1. Institute of Radiation Medicine, Fudan University, Shanghai 200032, China
    2. School of Stomatology, Lanzhou University, Lanzhou 730000, China
    3. Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China
    4. Dezhou City People's Hospital, Dezhou 253000, China
  • Received:2018-12-24 Revised:2019-02-27 Accepted:2019-02-27 Online:2019-08-20 Published:2019-08-21
  • Contact: Chunlin SHAO;
  • About author:HU Songling(female) was born in July 1987, and graduated from Lanzhou University in 2017. Now she is a doctoral candidate at Fudan University, majoring in radiation medicine. E-mail:
  • Supported by:
    the National Key R&D Program of China (2017YFC0108604) and National Natural Science Foundation of China(11179002)


To investigate the effects of X-rays and carbon-ion irradiation on CAL-27 cells and identify the differentially expressed microRNA (miRNA), CAL-27 cells were irradiated with X-rays and heavy-ions. Colony survival and apoptosis rate were measured. The differentially expressed miRNAs among different cell groups were obtained by high throughput sequencing technique and analyzed by Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis. After X-ray and heavy-ion irradiation, the survival rate of CAL-27 cells reduced to 88% and 71%, respectively and the rate of apoptosis increased from 1.9% in the control group to 13.9% and 30.4%, respectively. Compared with X-ray irradiated cells, 33 miRNAs were changed after heavy-ion irradiation, 12 of which were up-regulated and 21 were down-regulated. Fifteen pathways were further filtered out using the KEGG database. Compared with X-ray irradiation, heavy-ion irradiation had a greater effect on the expression of miRNAs in CAL-27 cells at the same dose. The mechanism may be related to the MAPK signal transduction pathways.

Key words: Heavy ion, CAL-27 cell, MicroRNA, Irradiation

CLC Number: 

  • Q691.5