Journal of Radiation Research and Radiation Processing ›› 2020, Vol. 38 ›› Issue (5): 50-59.doi: 10.11889/j.1000-3436.2020.rrj.38.050404


Study on a new technique for the treatment of Ganoderma lucidum spore powders using ball milling and γ ray irradiation

QI Hui,CHEN Liang,WU Xiaofen,ZHANG Yong,DENG Ming,WANG Keqin()   

  1. Hunan Institute of Nuclear Agricultural Science and Space Mutation Breeding, Hunan Academy of Agricultural Sciences, Changsha 410125, China
  • Received:2020-04-26 Revised:2020-06-04 Accepted:2020-06-04 Online:2020-10-20 Published:2020-10-19
  • Contact: WANG Keqin
  • About author:QI Hui (female) was born in December 1988, and obtained her master’s degree in quality of agricultural products and food safety from Chinese Academy of Agricultural Sciences in 2013. She is an assistant researcher engaging in the application of irradiation technology in agricultural
  • Supported by:
    Hunan Agricultural Science and Technology Innovation Fund Project(2019JG03)


To improve the wall-broken rate and polysaccharide content of Ganoderma lucidum spore powders, a combined treatment of γ ray irradiation and ball milling technique was used. Two-factor tests were conducted using the absorbed dose and the ball milling time as the test factors. The morphology and molecular structure of the Ganoderma lucidum spores were characterized using scanning electron microscope and Fourier transform infrared spectrometer tests under different treatment conditions. The results showed that when the absorbed dose was higher than 4.892 kGy, or the ball milling time was higher than 1 minute, the absorbed dose and ball milling time had significant effects on the wall-broken rate and the polysaccharide content of the Ganoderma lucidum spore powders, with the ball milling time having a greater effect. In addition, there was an interaction between the two pretreatment methods, which significantly affected the wall-broken rates and the polysaccharide contents of the Ganoderma lucidum spore powders. Furthermore, the optimal wall-broken rate of 97.18% and polysaccharide mass fraction of 13.230 mg/g were achieved when the Ganoderma lucidum spore powders were irradiated at 15.231 kGy with a ball milling time of 5 min. In addition, under these treatment conditions, the complete oval structure of the Ganoderma lucidum spores was almost completely broken. A small spike was observed at 1 456 cm-1, and a double peak was observed at 1 652 cm-1, which were completely different from the unbroken wall spores.

Key words: γ ray irradiation, Ball milling, Ganoderma lucidum spore powder, New technology of wall-broken

CLC Number: 

  • TL99