Journal of Radiation Research and Radiation Processing ›› 2020, Vol. 38 ›› Issue (5): 34-41.doi: 10.11889/j.1000-3436.2020.rrj.38.050402

• RADIATION TECHNOLOGY APPLICATION • Previous Articles    

Photosynthetic profiling and response to heavy-ion irradiation in Dunaliella salina

XI Yimei1,2,YIN Liang1,CHI Zhanyou2,LI Xin3,LUO Guanghong1()   

  1. 1.Hexi University, Gansu Microalgae Technology Innovation Center, Zhangye 734000, China
    2.School of Bioengineering, Dalian University of Technology, Dalian 116024, China
    3.Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China
  • Received:2020-04-07 Revised:2020-05-28 Accepted:2020-05-28 Online:2020-10-20 Published:2020-10-19
  • Contact: LUO Guanghong E-mail:kyluo@hxu.edu.cn
  • About author:XI Yimei (female) was born in June 1990, and graduated from Dalian Ocean University in 2014. Now she is a graduate student in Dalian University of Technology, engaging in microalgae synthetic biology, engaging in microalgae synthetic biology
  • Supported by:
    National Natural Science Foundation of China(11665011);Gansu Provincial Universities Collaborative Innovation and Technology Team Support Plan(2017C-17);Gansu Provincial Science and Technology Program(18JR2JG001)

Abstract:

In this study, we aimed to explore the biological effect and photosynthetic response mechanism of heavy-ion irradiation on Dunaliella salina. Dunaliella salina was treated with different doses (0~320 Gy) of heavy-ion irradiation.The cell biomass, chlorophyll fluorescence parameters, and main photosynthetic pigments of progenies from irradiated Dunaliella salina cells were systematically measured and analyzed within 10 days of treatment. The results showed a significant increase in the biomass of cells that underwent heavy-ion irradiation at 90 Gy, 240 Gy, and 320 Gy doses compared with non-irradiated Dunaliella salina cells. In particular, the maximum biomass of irradiated cells was 1.78-fold greater than of non-irradiated cells at 240 Gy. The chlorophyll fluorescence imaging system, Water-PAM, was used to analyze the chlorophyll fluorescence parameters, including photochemical efficiency of photosystem II (Fv/Fm), actual photosynthetic activity (ΦPSII), and non-photochemical quenching capacity (NPQ) of the cells after irradiation. The results indicated that 90 Gy, 240 Gy, and 320 Gy doses notably increased the photosynthetic efficiency of Dunaliella salina. The 240 Gy dose-irradiated algal cells exhibited activated photoprotection systems more rapidly as compared to cells irradiated at other doses. Additionally, the β-carotene content of the algal cells after irradiation was measured using UV spectrophotometry. The results revealed that β-carotene production increased compared with non-irradiated Dunaliella salina cells, by 1.3 and 1.1 times with 240 Gy and 320 Gy, respectively. To our knowledge, this study investigated the dose and time response to heavy-ion irradiation on Dunaliella salina mutagenesis for the first time, and provided a preliminary theoretical and experimental basis for the response mechanism of the photosynthetic system to heavy-ion irradiation.

Key words: Heavy-ion irradiation, Dunaliella salina, Photosynthetic efficiency, β-carotene

CLC Number: 

  • Q691