Journal of Radiation Research and Radiation Processing ›› 2020, Vol. 38 ›› Issue (4): 57-66.doi: 10.11889/j.1000-3436.2020.rrj.38.040401

• RADIATION TECHNOLOGY APPLICATION • Previous Articles    

Breeding of a highly effective organophosphorus-degrading mutant bacteria obtained by ion beam irradiation

PENG Yinan1,GONG Xiaofang1,ZHU Ying1,JI Bin1,LU Dong2,YANG Hui1,WANG Zhiye1()   

  1. 1.Key Laboratory of Microbial Resources Exploitation and Application of Gansu Province, Institute of Biology, Gansu Academy of Sciences, Lanzhou 730000, China
    2.Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China
  • Received:2020-02-18 Revised:2020-04-02 Accepted:2020-04-02 Online:2020-08-20 Published:2020-08-13
  • Contact: WANG Zhiye E-mail:zhiye_wang@sina.com
  • About author:PENG Yinan (female) was born in June 1988, and obtained her master's degree from Lanzhou University of Technology in 2013, majoring in biochemistry and molecular biology
  • Supported by:
    the Technology Program of Gansu Province(18JR3RA253);the Innovation Team Project of Gansu Academy of Sciences(2019CX004-01);the Application Development Project of Gansu Academy of Sciences(2017JK-11);the Outstanding Youth Fund of Gansu Academy of Sciences(2019QN-02)

Abstract:

An organophosphorus-degrading Bacillus subtilis strain was irradiated by 12C6+ ion beam and a highly efficient organophosphorus-degrading LA-1 strain was selected through selective breeding medium and shake flask experiments. The effect of organophosphorous pesticide degradation was also studied. The results showed that the highly stable LA-1 mutant strain was screened through eight generation propagation at an absorbed dose of 150 Gy. The amount of degraded organophosphorus was 161.71 mg/L, which was 2.89 times higher than that of the original strain. The degradation rates were 93.5% on phoxin and 83.6% on omethoate when the concentration reached 800 μg/mL and 400 μg/mL, respectively. The LA-1 mutant strain has a promising organophosphorus pesticide degradation rate.

Key words: Ion beam irradiation, Mutation breeding, Organophosphorus pesticide degradation, Bacillus subtilis

CLC Number: 

  • Q691