Journal of Radiation Research and Radiation Processing ›› 2020, Vol. 38 ›› Issue (2): 25-32.doi: 10.11889/j.1000-3436.2020.rrj.38.020402

• RADIATION TECHNOLOGY APPLICATION • Previous Articles     Next Articles

Screening temperature-tolerant and high-yield Spirulina maxima mutants using 12C6+ ion beam irradiation and optimizing its culture conditions

WANG Lijuan1,3,ZHENG Tianxiang2,YANG Songqi1,3,LI Xin4,LUO Guanghong1,3()   

  1. 1.Gansu Tech Innovation Center of Microalgae, Hexi University, Zhangye 734000, China
    2.College of Agriculture and Ecological Engineering, Hexi University, Zhangye 734000, China
    3.Key Laboratory of Hexi Corridor Resources Utilization of Gansu, Zhangye 734000, China
    4.Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China
  • Received:2019-07-15 Revised:2020-01-14 Accepted:2020-01-14 Online:2020-04-20 Published:2020-04-22
  • Contact: LUO Guanghong E-mail:13993693452@163.com
  • About author:WANG Lijuan (female) was born in October 1989, and obtained her master’s degree from Shenyang Pharmaceutical University in 2015. Now she is an assistant professor in Hexi University, engaging in the active components analysis of microalgae
  • Supported by:
    National Natural Science Foundation of China(11665011);Gansu Provincial Program on Supporting Scientific and Synergistic Innovation Team in Colleges and Universities(2017C-17);Gansu Provincial Scientific Research Projects for High Education Insitutions(2017A-086);Gansu Provincial Key Research and Development Project(18YF1WG087)

Abstract:

Spirulina maxima (S. maxima)was irradiated using 12C6+ ion beams at different doses, and the mutant strains were selected by plate separation and temperature treatment methods. Mutant strains with high biomass yields and increased temperature tolerance were screened out by genetic stability analysis. The culture conditions of the mutants were also optimized. Results showed that when the absorbed doses were 600 Gy and 800 Gy, the mutagenic effects on the algae strains were significant and the mortality rates were 71.84% and 78.47%, respectively. Two temperature-tolerant mutants (Sm04# and Sm21#) were obtained, which were cultured at 20 ℃ and 35 ℃ after irradiation at 600 Gy and 800 Gy. After ten generations, the biomasses of the mutants were 0.54 g/L and 0.91 g/L, respectively, which were 11.49% and 12.64% larger than the biomass of the control group, respectively. The culture conditions optimization experiment indicated that the optimum pH of both Sm04# and Sm21# was 9.0, the optimum light intensities were 70 and 80 μmol/(m2·s), respectively, and the optimum light-dark periods were 12:12 h and 14:10 h, respectively. In addition, the protein and polysaccharide contents of the two mutants were both higher than those of the control group, which provides a theoretical basis for the development and application of S. maxima using carbon ion irradiation.

Key words: Spirulina maxima, Carbon ion beam, Temperature tolerance, Culture conditions optimization

CLC Number: 

  • Q815