Journal of Radiation Research and Radiation Processing ›› 2020, Vol. 38 ›› Issue (2): 9-15.doi: 10.11889/j.1000-3436.2020.rrj.38.020301

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles     Next Articles

Radiological resistance regulatory mechanism of circRNA_017122 in bone marrow mesenchymal stem cells

ZHANG Junhua,HUANG Rong,CHEN Sai GUI Rong   

  1. Department of Blood Transfusion, The Third Xiangya Hospital of Central South University, Changsha 410000, China
  • Received:2019-11-21 Revised:2020-01-21 Accepted:2020-01-21 Online:2020-04-20 Published:2020-04-22
  • About author:ZHANG Junhua (female) was born in May 1984, and obtained her doctoral degree from Central South University in 2018. Now she is a technologist-in-charge at Department of Blood Transfusion, The Third Xiangya Hospital of Central South University
  • Supported by:
    National Natural Science Foundation of China(81573091);Natural Science Foundation of Hunan Province(2019JJ50909)

Abstract:

To investigate the radiological resistance regulatory mechanism of circRNA_017122 in bone marrow mesenchymal stem cells, qRT-PCR was used to detect mmu-circRNA_017122 expression in mouse bone marrow mesenchymal stem cells, following an irradiation treatment at different absorbed doses. The biological function of mmu-circRNA_017122 was predicted using TargetScan and miRanda software. Luciferase assay was used for the detection of mmu-circRNA_017122 and mmu-miR-29b-2-5p. Their binding was confirmed by RNA pull-down assay, while p53 expression and cell proliferation were detected by transfecting mmu-circRNA_017122 siRNA and mmu-miR-29b-2-5p inhibitors, respectively. The expression level of mmu-circRNA_017122 in bone marrow mesenchymal stem cells was affected by the absorbed dose. The biological software predicted that mmu-circRNA_017122 could specifically bind to mmu-miR-29b-2-5p and further affect P53 expression. Both the luciferase reporter assay and RNA pull-down confirmed this specific binding. After the simultaneous transfection of the mmu-circRNA_017122 siRNA and the mmu-miR-29b-2-5p inhibitor, both P53 expression and cell proliferation were significantly reduced. In irradiated bone marrow mesenchymal stem cells, the mmu-circRNA_017122 affected p53 expression by mmu-miR-29b-2-5p through a ceRNA mechanism, and thus participated in radiation damage repair, resulting in bone marrow mesenchymal stem cell radiosensitivity.

Key words: mmu-circRNA_017122, mmu-miR-29b-2-5p, P53, Radioresistance

CLC Number: 

  • Q691