Journal of Radiation Research and Radiation Processing ›› 2020, Vol. 38 ›› Issue (1): 42-49.doi: 10.11889/j.1000-3436.2020.rrj.38.010303

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles     Next Articles

Protective effect of compound raspberry seed powder on radiation-induced spinal cord injury in mice

LU Zhaoxiang1,WANG Jinhui1,XU Ning1,LUAN Jie1,PANG Xiangyu1,XIA Yujun2()   

  1. 1. Medical Department of Qingdao University, Qingdao 266071, China
    2. Teaching and Research Department of Human Anatomy and Histology and Embryology, Medical Department of Qingdao University, Qingdao 266071, China
  • Received:2019-08-12 Revised:2019-11-07 Accepted:2019-11-07 Online:2020-02-20 Published:2020-02-25
  • Contact: XIA Yujun E-mail:xiayujun62@163.com
  • About author:LU Zhaoxiang (male) was born in 1997. Now he is an undergraduate student at Department of Medicine, Qingdao University, majoring in clinical medicine

Abstract:

To observe the protective effect of compound raspberry seed powder on radiation induced spinal cord injury in mice. Fifty healthy adult male mice were randomly divided into five groups of ten: control group, model group, and different concentrations (low, medium, and high) of compound raspberry seed powder solution treatment group. The model group was given distilled water by gastric lavage and the treatment groups were given different concentrations of compound raspberry seed powder solution by gastric lavage. Except for the control group, the mice were irradiated with whole body X-ray at a dose of 4 Gy immediately after gastric administration on the 15th day after treatment. On the 3rd day after irradiation, the spinal cord tissues were taken for hematoxylin and eosin (HE) and Nissl staining to observe morphological changes and the expression of Fas and caspase-9 in spinal cord tissues was detected by immunohistochemistry(IHC). HE staining showed swelling and disorder of nerve fibers in the white matter of the spinal cord, atrophy, and disappearance of some neurons in gray matter, accompanied by an inflammatory reaction. Nissl staining showed that the Nissl body dissolved and neurons disappeared in the model group. IHC showed that the staining degree of Fas and caspase-9 in the model group was significantly higher than that in the control group (p<0.01). The staining degree of Fas and caspase-9 in compound raspberry powder solution treatment groups was lower than that in the model group and gradually decreased with the increasing concentrations(p<0.05). Compound raspberry seed powder can alleviate radiation-induced spinal cord injury and inhibit the overexpression of Fas and caspase-9 to inhibit apoptosis. With an increase in concentration, the protective effect is more obvious.

Key words: Raspberry, Radiation-induced spinal cord injury, Radiation protective agent, Apoptosis

CLC Number: 

  • R81,TL71