Journal of Radiation Research and Radiation Processing ›› 2020, Vol. 38 ›› Issue (1): 23-31.doi: 10.11889/j.1000-3436.2020.rrj.38.010301

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles     Next Articles

Effect of silencing human acetytransferase-like protein on regulation of radiosensitivity in colorectal cancer CL187 cells

HU Lelin1,2(),TAO Huihui3,YANG Yangli1,TAO Xinrong3,TANG Xiaolong3   

  1. 1. First Clinical Hospital, School of Medicine, Anhui University of Science and Technology, Huainan 232001, China
    2. Department of Radiation Oncology, The First Affiliate Hospital of Anhui University of Science and Technology, Huainan 232001, China
    3. Department of Cell Biology, School of Medicine, Anhui University of Science and Technology, Huainan 232001, China
  • Received:2019-06-24 Revised:2019-09-02 Accepted:2019-09-02 Online:2020-02-20 Published:2020-02-25
  • About author:HU Lelin (female) was born in September 1979, and obtained her doctoral degree of oncology from Peking University. Now she is a physicist in Department of Radiation Oncology, the First Affiliated Hospital of Anhui Science and Technology University. E-mail: hulelin1979@163.com
  • Supported by:
    the Natural Science Research Project of Education Department of Anhui Province(KJ2018A0096);Initiation Fund of Scientific Research for Introducing Talent of Anhui University of Science and Technology, and National Undergraduate Training Programs for Innovation and Entrepreneurship(201910361116)

Abstract:

To investigate the effect of human acetytransferase-like protein(hALP) SiRNA on radiosensitivity of colorectal cancer CL187 cells. For this, the expression of hALP in colon cancer tissues was investigated, based on information available in clinical databases. After hALP knockdown, CL187 cells were irradiated with gradually increasing doses(0 Gy,2 Gy,4 Gy,6 Gy and 8 Gy), and clonal proliferation and cell viability assays were used to detect radiosensitivity. After with the hALP SiRNA duplex, the percentage of cells in which apoptosis was induced by radiation at the dose of 8 Gy was detected by flow cytometric analysis, and the expression of hALP, P53, and BAX was examined by western blotting. Following irradiation, the ratios of surviving fraction between hALP SiRNA and negative control SiRNA groups were 0.63, 0.5, respectively, for total doses of 2 Gy(RBE=1.26). Silencing hALP enhanced the radiosensitivity of CL187 cells. Moreover, hALP knockdown increased the percentage of CL187 cells undergoing apoptosis from 19.53% to 36.49%(p<0.05). The expression of hALP, P53 and BAX induced by radiation further increased after hALP knockdown expression. Further, hALP knockdown expression increased radiosensitivity of CL187 cells. The expression of hALP associated with the radiosensitivity of colorectal cancer cells, which is beneficial to screen potential molecular targets and to provide indispensable experimental data for improving the efficacy of radiotherapy.

Key words: SiRNA, hALP, Colorectal cancer, Radiosensitivity

CLC Number: 

  • R735.3