Journal of Radiation Research and Radiation Processing ›› 2019, Vol. 37 ›› Issue (6): 26-33.doi: 10.11889/j.1000-3436.2019.rrj.37.060302

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles     Next Articles

Quercetin promotes apoptosis in ionizing radiation-induced senescent cells

LONG Kaiqin1,2,HE Jinpeng2,ZHANG Tongshan2,3,MA Wei2,3,HUA Junrui2,WANG Jufang2()   

  1. 1. School of Pharmacy, Lanzhou University, Lanzhou 730000, China
    2. Key Laboratory of Space Radiobiology of Gansu Province, Institute of Modern Physics, Chinese Academy of Science, Lanzhou 730000, China
    3. University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2019-08-28 Revised:2019-10-12 Accepted:2019-10-12 Online:2019-12-20 Published:2019-12-23
  • Contact: WANG Jufang E-mail:jufangwang@impcas.ac.cn
  • About author:LONG Kaiqin(female) was born in September 1993, and graduated from Sichuan University in 2017. Now she is a master candidate at Lanzhou University, majoring in radiation pharmacology
  • Supported by:
    National Natural Science Foundation of China(U1232125);Gansu Science and Technology Program Project(145RTSA012)

Abstract:

The aim of this study was to investigate the effect of quercetin on apoptosis and the expression of apoptosis-related proteins in human uveal melanoma 92-1 cells exposed to X-ray (10 Gy) or carbon-ion irradiation. Irradiated 92-1 cells were treated with quercetin, and then, cellular apoptosis and senescence were measured by Annexin V-FITC/PI assay with flow cytometry and senescence-associated β-galactosidase (SA-β-Gal) staining, respectively. To confirm the appropriate concentration of quercetin to be used, cell viability was assessed by the cell counting kit-8 (CCK-8) assay and apoptosis was detected. The expression of cleaved caspase-3 was detected by western blotting. We found that 92-1 cells developed an obvious senescence phenotype, and cell cultures showed a low proportion of cells undergoing apoptosis after exposure to X-ray or carbon-ion irradiation. Treatment with 50 μmol/L quercetin immediately, or 3 d after irradiation, induced significant apoptosis and upregulated the expression of cleaved caspase-3. These results demonstrate that quercetin induces apoptosis in 92-1 cells after X-ray or carbon-ion irradiation.

Key words: Ionizing radiation, Cell senescence, Quercetin, Apoptosis

CLC Number: 

  • R96