Journal of Radiation Research and Radiation Processing ›› 2019, Vol. 37 ›› Issue (6): 18-25.doi: 10.11889/j.1000-3436.2019.rrj.37.060301

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles     Next Articles

Study on radio-sensitive proteins to ionizing radiation in mice serum

BAI Hao1,2,WEI Wenjun1,WANG Jufang1(),MA Wei1,2   

  1. 1. Key Laboratory of Space Radiobiology of Gansu Province,Institute of Modern Physics,Chinese Academy of Sciences,Lanzhou 730000,China
    2. University of Chinese Academy of Sciences,Beijing 100049,China
  • Received:2019-09-23 Revised:2019-10-22 Accepted:2019-10-22 Online:2019-12-20 Published:2019-12-23
  • Contact: WANG Jufang E-mail:jufangwang@impcas.ac.cn
  • About author:BAI Hao(male) was born in September 1996, and graduated from Sichuan University in 2018. Now he is a master candidate at the Institute of Modern Physics, Chinese Academy of Sciences, majoring in biophysics
  • Supported by:
    the Strategic Priority Research Program of Chinese Academy of Science & Pre-research Program of Space Science(XDA15014400);the National Natural Science Foundation of China(11805246);the Science and Technology Research Project of Gansu Province(145RTSA012)

Abstract:

This study aimed to identify serum protein responses to ionizing radiation and to indicate their capacity for serving as radiation biomarkers. We performed antibody-based protein microarray studies to determine the expression profiles of serum proteins in mice 24 h after varying doses of X-ray irradiation. A set of 47 serum proteins with relative increases in expression of ≥1.5 fold was identified. Among them, insulin-like growth factors 1 and 2 (IGF-1 and IGF-2), and insulin-like growth factor binding proteins 1 and 3 (IGFBP-1 and IGFBP-3) had higher abundance and were reported to be involved in radiation-induced DNA damage and repair through the IGF-IGFBP axis. Therefore, these four proteins and their upstream regulatory protein-growth factor (GH) were selected for further validation and assessment of their dose and time dependency by enzyme-linked immune sorbent assay. The results revealed that levels of serum GH and IGF-1 were not significantly different from those in the control group (0 Gy) after 0.5, 2, and 4 Gy of X-ray irradiation, while levels of serum IGF-2, IGFBP-1, and IGFBP-3 showed an upward trend. Levels of IGFBP-3 were significantly increased, with an obvious dose-dependence (p<0.01). Moreover, between 2 and 24 h post-irradiation, expression of serum GH fluctuated greatly, while IGF-1 showed no significant changes, and levels of IGF-2, IGFBP-1, and IGFBP-3 increased. The upward trends in IGFBP-1 and IGFBP-3 expression were evident from 2 to 24 h, at least. These results demonstrated that levels of serum IGF-2, IGFBP-1, and IGFBP-3 showed significant increases with good dose and time dependent effects after X-ray irradiation, suggesting that these proteins have great potential as biomarkers of ionizing radiation.

Key words: Ionizing radiation, Biomarkers, Serum, Insulin-like growth factors, Bindin

CLC Number: 

  • Q691