Journal of Radiation Research and Radiation Processing ›› 2019, Vol. 37 ›› Issue (5): 5-050301-8.doi: 10.11889/j.1000-3436.2019.rrj.37.050301

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles     Next Articles

Application of cell-free filtrate of Pleurotus geesteranus mycelium: biosynthesis of silver nanoparticles and their antimicrobial activity

DONG Lina1(),LIU Xuerui1,WANG Huijie2,WANG Lei2,XU Hui1(),ZHOU Yudi3   

  1. 1. College of Chemistry and Materials Science, Ludong University, Yantai 264025, China
    2. College of Life Sciences, Ludong University, Yantai 264025, China
    3. Shanghai HuaEr Private Middle School, Shanghai 200122, China
  • Received:2019-05-06 Revised:2019-07-02 Accepted:2019-07-02 Online:2019-10-20 Published:2019-10-15
  • Contact: Hui XU;
  • About author:DONG Lina(female) was born in October 1995, and obtained her bachelor’s degree from Heze University in 2018. Now she is a master candidate at Ludong University, majoring in material science. E-mail:
  • Supported by:
    the Key Research and Development Program of Shandong Province of China(2019GSF111047)


This study evaluated the in vitro antibacterial activity of silver nanoparticles (AgNPs) synthesized from the cell-free filtrate of Pleurotus geesteranus. AgNPs were characterized by ultraviolet-visible absorption spectroscopy, X-ray diffraction, transmission electron microscopy, and zeta potential. The antibacterial activity of the AgNPs was evaluated by the agar diffusion method against Vibrio anguillaru, Vibrio alginolyticus, Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Pseudomonas aeruginosa. Escherichia coli was selected as the index bacteria to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the AgNPs. Well-dispersed spherical or nearly spherical AgNPs were obtained with particle sizes ranging from 4.5 to 18.2 nm. The acquired AgNPs effectively inhibited the growth of various bacteria. The MIC and MBC were 8.2 μg/mL and 32.5 μg/mL, respectively. The controllable synthesis of AgNPs achieved using the cell-free filtrate of P. geesteranus produced AgNPs that exhibited excellent broad-spectrum antibacterial activity.

Key words: Silver nanoparticles, Biosynthesis, Pleurotus geesteranus, Antibacterial activity

CLC Number: 

  • TL13