Journal of Radiation Research and Radiation Processing ›› 2019, Vol. 37 ›› Issue (4): 8-040701-7.doi: 10.11889/j.1000-3436.2019.rrj.37.040701

• RADIATION INTERDISCIPLINARY RESEARCH • Previous Articles     Next Articles

Detecting γ-radiation damage of DNA-AuNPs assembliesusing surface-enhanced Raman scattering

CHEN Yu WANG Kaikai ZHANG Haiqian()   

  1. College of Materials Science and Technology, Nanjing University of Aeronautics and Astronautics, Nanjing 211106, China
  • Received:2019-01-18 Revised:2019-03-20 Accepted:2019-03-20 Online:2019-08-20 Published:2019-08-21
  • Supported by:
    National Natural Science Foundation of China(11575086)

Abstract:

A novel approach for detecting radiation damage to radiosensitive genes was developed using surface-enhanced Raman scattering (SERS) technology. Radiosensitive gene fragments were modified with thiol groups and immobilized onto the surface of gold nanoparticles (AuNPs) to form single-stranded and double-stranded DNA through Au-S bonds. SERS was used to analyze the response of DNA-AuNPs assemblies to γ-rays and the local enhancement effect of AuNPs on this response to γ-radiation. The results showed that the 663 cm-1 spectral peak of the double-stranded DNA-AuNPs assemblies varied with the dose range of γ-radiation (0 to ~200 Gy). Similarly, the single-stranded DNA-AuNPs showed a good response to γ-radiation in the dose range of 0~100 Gy, and the sensitivity of the DNA-AuNPs assembly noticeably increased with the increase in bound AuNPs. This positive correlation was due to the localized surface plasmon resonance of the AuNPs. The analysis of DNA-AuNPs assemblies using SERS scattering, could potentially be a novel method for the detection and measurement of ionizing radiation causing damage to genes.

Key words: Radiosensitive gene, γ-radiation, Surface enhanced Raman scattering, Gold nanoparticles

CLC Number: 

  • TL816+.2