Journal of Radiation Research and Radiation Processing ›› 2019, Vol. 37 ›› Issue (4): 6-040305-7.doi: 10.11889/j.1000-3436.2019.rrj.37.040305

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles     Next Articles

Effect of cimetidine on spleen cell apoptosis and expression of apoptosis-related proteins in Beagle dogs exposed to low-dose-rate radiation

WANG Qingrong HE Ying ZHAO Yining SHEN Xianrong LIU Yuming LI Kexian()   

  1. Department of Radiation Protection Medicine, Naval Medical Research Institute, The Second Military Medical University, Shanghai 200433, China
  • Received:2018-12-10 Revised:2019-04-15 Accepted:2019-04-15 Online:2019-08-20 Published:2019-08-21
  • Supported by:
    the Ministry Special Drugs Project of National Major Scientific and Technological Special Project for Significant New Drugs Development(2014ZX09J14103-07B)

Abstract:

The aim of this study was to investigate the effect of cimetidine on spleen cell apoptosis and the expression of apoptosis-related proteins in Beagle dogs exposed to low-dose-rate radiation and to explore its mechanism of radioprotection. Twenty-four male Beagle dogs were divided into six groups: normal control group, model group, positive control group, and cimetidine-treated groups at doses of 5.33, 10.67, and 21.33 mg/kg. The dogs were irradiated with 60Co γ-rays at a rate of 0.040 8 mGy/min for 23 d. Cimetidine was administered intragastrically once daily during the irradiation period. At cumulative dose of 991.44 mGy, the apoptosis of spleen cells was observed by the TUNEL assay, and the expression of Bcl-2, Bax, and P53 in the spleen was detected by immunohistochemistry. A high dose of cimetidine significantly inhibited spleen cell apoptosis induced by irradiation. In addition, the expression of Bcl-2 in the spleen increased in the cimetidine groups. Moreover, the expression of Bax, caspase-3, and P53 was reduced and the change was statistically significant in the cimetidine groups. Cimetidine induced the expression of Bcl-2 and reduced the expression of Bax, caspase-3, and P53 in the spleen cells of Beagle dogs, thereby inhibiting apoptosis and protecting the spleen from cumulative exposure to low-dose-rate radiation.

Key words: Cimetidine, Low-dose-rate radiation, Cell apoptosis, Spleen

CLC Number: 

  • R965