Journal of Radiation Research and Radiation Processing ›› 2019, Vol. 37 ›› Issue (4): 3-040302-8.doi: 10.11889/j.1000-3436.2019.rrj.37.040302

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles     Next Articles

Apoptotic effect of wide-band electromagnetic pulse on epithelial cells

YANG Yu WANG Xianghui QI Hongxing ZHANG Jie   

  1. Biophysics Laboratory, School of Physics and Materials Science, East China Normal University, Shanghai 200241, China
  • Received:2018-12-12 Revised:2019-02-25 Accepted:2019-02-25 Online:2019-08-20 Published:2019-08-21
  • Supported by:
    National Natural Science Foundation of China(31600675)

Abstract:

The purpose of this study was to investigate the relationship between cell apoptosis and wide-band electromagnetic pulse (WEMP) irradiation parameters and to provide experimental data to establish the biophysics damage model for WEMP. Cell counting kit-8 was used to detect the cell viability changes after WEMP irradiation and flow cytometry assay was used to detect cell apoptosis after irradiation under different WEMP irradiation parameters. Under experimental conditions, field strength and number of pulses were observed to be the main factors that increased the apoptotic rate of human amniotic epithelial cells. Specifically, electric field intensity and number of pulses were determined to be the primary and secondary factors, respectively. The two factors were confirmed to interact with each other, that is, a logistic function was found to exist between the apoptotic rate and electric field strength.

Key words: Wide-band electromagnetic pulse,Apoptosis,Variance analysisCLC, TL71, Q689

CLC Number: 

  • TL71,Q689