宽频电磁脉冲辐照对上皮细胞凋亡损伤的影响

doi:10.11889/j.1000-3436.2019.rrj.37.040302

Journal of Radiation Research and Radiation Processing ›› 2019, Vol. 37 ›› Issue (4): 3-040302-8.doi: 10.11889/j.1000-3436.2019.rrj.37.040302

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles Next Articles

Apoptotic effect of wide-band electromagnetic pulse on epithelial cells

YANG Yu WANG Xianghui QI Hongxing ZHANG Jie

Biophysics Laboratory, School of Physics and Materials Science, East China Normal University, Shanghai 200241, China

Received:2018-12-12 Revised:2019-02-25 Accepted:2019-02-25 Online:2019-08-20 Published:2019-08-21
Supported by:
National Natural Science Foundation of China(31600675)

Abstract

Abstract:

The purpose of this study was to investigate the relationship between cell apoptosis and wide-band electromagnetic pulse (WEMP) irradiation parameters and to provide experimental data to establish the biophysics damage model for WEMP. Cell counting kit-8 was used to detect the cell viability changes after WEMP irradiation and flow cytometry assay was used to detect cell apoptosis after irradiation under different WEMP irradiation parameters. Under experimental conditions, field strength and number of pulses were observed to be the main factors that increased the apoptotic rate of human amniotic epithelial cells. Specifically, electric field intensity and number of pulses were determined to be the primary and secondary factors, respectively. The two factors were confirmed to interact with each other, that is, a logistic function was found to exist between the apoptotic rate and electric field strength.

Key words: Wide-band electromagnetic pulse,Apoptosis,Variance analysisCLC, TL71, Q689

CLC Number:

TL71，Q689

YANG Yu WANG Xianghui QI Hongxing ZHANG Jie. Apoptotic effect of wide-band electromagnetic pulse on epithelial cells[J].Journal of Radiation Research and Radiation Processing, 2019, 37(4): 3-040302-8.

Figures/Tables 5

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Table 1

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电场强度	细胞凋亡率 Apoptosis rate / %
Electric field intensity / kV·m^－1	N=2	N=5	N=50	N=100
对照组 Control group	5.21±0.28	2.74±1.49	4.02±0.21	4.13±1.74
假辐照组 Sham group	5.04±0.25	3.63±2.12	3.83±1.11	4.36±0.61
E=50	–	–	–	3.65±0.09
E=62	–	–	–	3.74±0.51
E=83	–	4.29±1.69	3.16±0.73	3.90±0.72
E=125	–	4.59±1.17	4.11±0.73	4.03±0.40
E=130	5.07±3.27	4.59±0.41	3.80±1.78	4.28±0.52
E=216	5.68±2.84	7.28±2.51*	6.74±3.70*	6.94±0.38**
E=384	8.12±0.44	7.78±0.77**	10.39±1.14**	10.53±0.81**
E=615	7.14±1.62	7.77±0.64**	9.94±1.69**	10.56±1.22**
E=862	7.23±0.32	7.37±0.93**	9.02±0.90**	13.11±2.77**
E=1 000	7.97±1.28	9.26±1.39**	12.32±1.27**	13.04±0.53**

源 Sourse	III类平方和 III type sum of suqare	自由度 Degree of freedom	均方 Mean square	F检验 F test	p值 p value	偏η平方 Partial η square
修正模型 Corrected model	892.245	35	25.493	10.945	0.000	0.842
截距 Intercept	3 866.426	1	3 866.426	1 659.949	0.000	0.958
场强 Electric field intensity	747.221	10	74.722	32.080	0.000	0.817
脉冲个数 Pulse number	56.703	3	18.901	8.115	0.000	0.253
电场强度×脉冲个数 Electric field intensity×pulse number	135.405	22	6.155	2.642	0.001	0.447
误差 Error	167.706	72	2.329	–	–	–
总计 Total	6 076.745	108	–	–	–	–
修正后总计 Corrected total	1 059.950	107	–	–	–	–

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Apoptotic effect of wide-band electromagnetic pulse on epithelial cells

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