Journal of Radiation Research and Radiation Processing ›› 2019, Vol. 37 ›› Issue (3): 4-030301-9.doi: 10.11889/j.1000-3436.2019.rrj.37.030301

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles     Next Articles

Protective effects of Sipunculus nudus polysaccharide on radiation damage in human intestinal epithelial cells

GAO Chunli1,2(),SHI Te3(),ZHANG Junling4,SHEN Xianrong2,HE Ying2,WANG Qingrong2()   

  1. 1. College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China
    2. Naval Medical Research Institute, The Second Military Medical University, Shanghai 200433, China
    3. College of Basic Medical, The Second Military Medical University, Shanghai 200433, China
    4. Institute of Radiation Medicine, Fudan University, Shanghai 200032, China
  • Received:2018-11-07 Revised:2018-12-06 Accepted:2018-12-06 Online:2019-06-20 Published:2019-06-18
  • Contact: Qingrong WANG E-mail:17612107936@163.com;qingrongw@126.com
  • About author:GAO Chunli (female) was born in November 1991, and obtained her bachelor’s degree from Qujing Normal University of Yunnan Province in 2016. Now she is a master’s candidate at Shanghai Ocean University. E-mail:17612107936@163.com; co-SHI Te (female) was born in June 1996, undergraduate student, will graduate from Second Military Medical University in 2019. E-mail: 872243146@qq.com|GAO Chunli (female) was born in November 1991, and obtained her bachelor’s degree from Qujing Normal University of Yunnan Province in 2016. Now she is a master’s candidate at Shanghai Ocean University. E-mail:17612107936@163.com; co-SHI Te (female) was born in June 1996, undergraduate student, will graduate from Second Military Medical University in 2019. E-mail: 872243146@qq.com|GAO Chunli (female) was born in November 1991, and obtained her bachelor’s degree from Qujing Normal University of Yunnan Province in 2016. Now she is a master’s candidate at Shanghai Ocean University. E-mail:17612107936@163.com; co-SHI Te (female) was born in June 1996, undergraduate student, will graduate from Second Military Medical University in 2019. E-mail: 872243146@qq.com
  • Supported by:
    Supported by National Natural Science Foundation of China(11635014)

Abstract:

To study the protective effects of Sipunculus nudus polysaccharide (SNP) on human intestinal epithelial cell (HIEC) damage induced by ionizing radiation, the survival rate of HIECs was detected by CCK-8 method, and the concentration of SNP was identified. The effect of SNP on the proliferation ability of post-irradiated HIECs was analyzed by a cloning method. After 137Cs γ-ray irradiation for 48 h with the absorbed dose of 10 Gy, cell apoptosis was observed by microscopy using the Hoechst33342/PI staining, and the apoptosis rate and ROS levels were measured using flow cytometry. The results showed that the HIEC survival rate decreased significantly with the increasing absorbed doses. When HIECs were pretreated with a certain concentration of SNP, the survival rate increased significantly, showing a positive correlation with the concentration of SNP, and reached the maximum level when the SNP mass concentration was 50 μg/mL. After irradiation, the survival rate of the cloned cells was observed to be higher than that of irradiation model group. The apoptosis rate and intracellular ROS levels decreased significantly for HIECs pretreated with SNP as compared with the irradiation model group. These results indicated that the SNP pretreatment could protect HIECs from damage caused by radiation.The underlying mechanism might be attributed to free radical scavenging by SNP and reduction in cell apoptosis.

Key words: Sipunculus nudus polysaccharide, Human intestinal epithelial cell, Radiation damage, Cell apoptosis

CLC Number: 

  • R931