Journal of Radiation Research and Radiation Processing ›› 2019, Vol. 37 ›› Issue (2): 20401-020401.doi: 10.11889/j.1000-3436.2019.rrj.37.020401

• RADIATION TECHNOLOGY APPLICATION • Previous Articles     Next Articles

Influence of electron-beam irradiation on amino acids and protein structure

GU Kefei1,2,3(),CHEN Zhijun4,5()   

  1. 1. Institute for Agri-food Quality Standards and Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
    2. Agricultural Products Quality and Safety Risk Assessment Laboratory Ministry of Agriculture and Rural Affaies of the People's Republic of China, Shanghai 201403, China
    3. Supervision and Testing Center for Edible Fungi Quality, Ministry of Agriculture and Rural Affaies of the People's Republic of China, Shanghai 201403, China
    4. Institute of Crop Science, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
    5. Shanghai SN Irradiation Technology Co., Ltd. Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
  • Received:2018-09-12 Revised:2018-11-20 Accepted:2018-11-20 Online:2019-04-20 Published:2019-04-19
  • Contact: Zhijun CHEN E-mail:xyz694@163.com;13764023962@126.com
  • About author:<named-content content-type="corresp-name">GU Kefei</named-content> (female) was born in October 1977, and received her master’s degree from Graduate School of Chinese Academy of Agricultural Sciences in 2007. Now she works at Institute for Agri-food Quality Standards and Technology, Shanghai Academy of Agricultural Sciences as an assistant professor, focusing on food nutrition and safety control. E-mail: <email>xyz694@163.com</email>|CHEN Zhijun, associate professor, E-mail: <email>13764023962@126.com</email>
  • Supported by:
    Supported by the Risk of National Ministry of Agriculture of Agricultural Products Quality Safety Evaluation Project "edible fungi products nutrient and quality assessment and critical control point (GJFP201700604)", the Shanghai Municipal Agricultural Commission, P. R. China "Study on the Role and Mechanism of Electron Beam Irradiation in the Control of Food Pathogens"(2016 Hu Nong Ke Gong Zi No.6-3-4)

Abstract:

In order to explore the influence of irradiation on the nutritional value of protein and amino acids in agricultural products, the effect of electron-beam irradiation on the structure of 17 amino acids contained within bovine serum albumin (BSA) was analyzed by mass spectrometry and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Electron-beam irradiation of BSA significantly influenced content of methionine and of the other 8 essential amino acids. An absorbed dose of 1 kGy decreased the mass concentration of methionine by 65%. Turbidity of aqueous BSA solution increased concomitantly with increasing absorbed dose. This was due to irradiation-induced BSA cross-linking and degradation, as demonstrated by SDS-PAGE. Mass spectrometry analysis revealed various secondary BSA structures (irregular, curl, bend, and corner) and demonstrated susceptibility of two sulfur bonds in the amino acid chain to radiation.

Key words: Amino acids, Bovine serum albumin, Electron-beam irradiation, Structure

CLC Number: 

  • TR699,TL99