Journal of Radiation Research and Radiation Processing ›› 2019, Vol. 37 ›› Issue (1): 10401-010401.doi: 10.11889/j.1000-3436.2019.rrj.37.010401

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Carbon ion-beam irradiation screening of a butanol-tolerant mutant of Clostridium acetobutylicum

GAO Yue1,2(),YANG Yang3,ZHANG Miaomiao1,2,GUO Xiaopeng1,2,LI Wenjian1,LU Dong1()   

  1. 1. Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China
    2. University of Chinese Academy of Sciences, Beijing 100049, China
    3. School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China
  • Received:2018-09-03 Revised:2018-11-16 Accepted:2018-11-16 Online:2019-02-20 Published:2019-01-30
  • Contact: Dong LU E-mail:gaoyue@impcas.ac.cn;ld@impcas.ac.cn
  • About author:GAO Yue (female) was born in October 1992, and graduated with a bachelor’s degree from Tianjin University of Science & Technology in 2016. Now she is a doctoral candidate at Institute of Modern Physics, Chinese Academy of Sciences, majoring in radiation biology. E-mail: gaoyue@impcas.ac.cn|Ph.D. LU Dong, associate professor, E-mail:ld@impcas.ac.cn
  • Supported by:
    Supported by the Key Deployment Projects of the Chinese Academy of Sciences (KFZD-SW-109), and CAS-ITRI cooperation program(CAS-ITRI201801)

Abstract:

To improve butanol tolerance of Clostridium acetobutylicum strains, 12C6+ ion-beam irradiation screening identified a C. acetobutylicum mutant (G01) with high productivity and resistance to butanol stress. Compared with the wild type C. acetobutylicum CICC 8012, mutant G01 was resistant to 19 g/L butanol and exhibited a 33% higher butanol production. The results indicate that 12C6+ ion beam irradiation can increase butanol tolerance and productivity of C. acetobutylicum. These data provide a reference for further culture and development of industrially relevant strains of C. acetobutylicum that have higher butanol tolerance and productivity.

Key words: 12C6+ ion beam, Irradiation mutation, Butanol fermentation, Butanol tolerance

CLC Number: 

  • Q691.5,TL99