Journal of Radiation Research and Radiation Proces ›› 2018, Vol. 36 ›› Issue (4): 40203-040203.doi: 10.11889/j.1000-3436.2018.rrj.36.040203

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles     Next Articles

Radioprotective effects of Angelica sinensis protein on mice

LI Xian1, WANG Xiangling1, HE Huocong2, LI Lingling1, CHEN Cuihuang1, PAN Jianru1   

  1. 1. College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, China;
    2. Laboratory of Radiation Oncology and Radiobiology, Fujian Cancer Hospital & Fujian Medical University Cancer Hospital, Fuzhou 350014, China
  • Received:2018-04-26 Revised:2018-06-04 Online:2018-08-20 Published:2018-08-18
  • Supported by:

    Supported by National Natural Science Foundation of China (81472907), and Natural Science Foundation of Fujian Province (2018J01732)

Abstract:

Protein named AP with molecular weight of 18.33 kDa was purified from prepared slices of Angelica sinensis using 80% ammonium sulfate precipitation and Sephadex G-50 gel filtration chromatography. A mouse model of acute radiation injury was then established via whole-body irradiation with X-rays at a dose of 6 Gy. The radioprotective effects of Angelica sinensis protein AP were evaluated using amifostine as a control by measuring body weight, peripheral blood leukocyte count, thymus index, spleen index, colony forming unit-spleen (CFU-S), morphology and histopathology of the spleen, and splenic antioxidant enzyme activity in the irradiated mice. The results showed that AP significantly increased the number of peripheral blood leukocytes, thymus index, spleen index, CFU-S, and catalase activity in irradiated mice by 135%, 103%, 23%, 219%, and 180%, respectively, and kept the number of splenic white pulp, resulting in a significant 94% reduction in weight loss. These results indicate that the Angelica sinensis protein AP has radioprotective effects on mice.

Key words: Angelica sinensis protein, X-rays, Radiation protection, Mice

CLC Number: 

  • R852.7