Journal of Radiation Research and Radiation Proces ›› 2018, Vol. 36 ›› Issue (4): 40202-040202.doi: 10.11889/j.1000-3436.2018.rrj.36.040202

• RADIOBIOLOGY AND RADIOMEDICINE • Previous Articles     Next Articles

Effects of electromagnetic pulse exposure on development of testicle in the growing male mice

LIN Yanyun1, WANG Yafeng2, GAO Peng1, GUO Qiyan1, HU Junfeng1, LIU Junye1, GUO Guozhen1, LANG Haiyang1, ZENG Lihua1   

  1. 1. Department of Radiation Medicine, Faculty of Preventive Medicine, Airforce Military Medical University, Xi'an 710032, China;
    2. Health and Epidemic Prevention Team, Navy General Hospital, Beijing 101113, China
  • Received:2018-03-30 Revised:2018-06-04 Online:2018-08-20 Published:2018-08-18
  • Supported by:

    Supported by National Natural Science Foundation of China (51677189, 51437008), and Natural Science Foundation of Shaanxi Province (2014KTCQ03-02)

Abstract:

Ninety-six mice aged 3 weeks were randomly divided into two groups:a group exposed to electromagnetic pulse (EMP) at 200 kV/m in 400 pulses for 30 d, 2 h/d and a sham-exposure group. The body weights were measured during the exposure time. The testicular index; sperm count, sperm deformity rate, spermatogenic cells apoptosis; and metastasis tumor antigen 1 (MTA-1) protein and mRNA expression in testicles were measured at 1-, 7-, 14-, 21-, 30-, and 60-day time points after exposure. The results showed that body weights in the exposure group were significantly lower than those in the sham-exposure group on days 8~28 of EMP exposure (p<0.05); compared with that in the sham-exposure group, testicular index in the exposure group decreased significantly on days 21~60 after exposure; sperm count decreased on days 7~60 after exposure; the rate of sperm deformation increased on days 1~30 after exposure; apoptotic spermatogenic cells increased on days 1 and 60 but decreased on days 7 and 14 after exposure (p<0.05); MTA-1 protein expression in testicles decreased significantly at all time points after exposure; and mRNA expression decreased on days 1~21 after exposure. The reduced testicular indexes and sperm quality, as measured by the decreases in MTA-1 protein and mRNA, suggest that EMP exposure inhibits the development of testicles in growing male mice.

Key words: Electromagnetic pulse (EMP), BALB/c mice, Testicle, Sperm, Metastasis tumor antigen 1 (MTA-1)

CLC Number: 

  • Q64