Journal of Radiation Research and Radiation Proces ›› 2018, Vol. 36 ›› Issue (4): 40201-040201.doi: 10.11889/j.1000-3436.2018.rrj.36.040201

• RADIOBIOLOGY AND RADIOMEDICINE •     Next Articles

Radioprotective effect of beta-D-glucan derived from Saccharomyces cerevisiae on human umbilical vein endothelial cells

LIU Fang1,2, WANG Zhuanzi1, LI Wenjian1, WEI Wei1, DANG Bingrong1   

  1. 1. Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;
    2. University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2018-04-19 Revised:2018-05-12 Online:2018-08-20 Published:2018-08-18
  • Supported by:

    Supported by National Natural Science Foundation of China (11575259)

Abstract: To explore the radioprotective effect of Saccharomyces cerevisiae-derived-beta-D-glucan (BG) on human umbilical vein endothelial cells (Eahy926), cytotoxicity of BG on Eahy926 cells was detected by 3-(4, 5- Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), and the optimum concentration and pretreatment time were confirmed (10 ?g/mL, 24 h). The clone formation assay was used to detect the BG effect on radiosensitivity of Eahy926 cells. At 0.5 h and 24 h after X-ray irradiation at a absorbed dose of 2 Gy, the apoptosis, DNA damage, and intracellular reactive oxygen species (ROS) of Eahy926 cells in each group were measured through flow cytometry, and the enzyme activities of superoxide dismutase (SOD) and catalase (CAT) were also detected. The results showed that BG was non-toxic to Eahy926 cells. BG pretreatment could decrease the cell damage caused by X-rays and significantly increase clone survival rates. At 0.5 h and 24 h after irradiation, the DNA damage, cell apoptosis rates, and intracellular ROS level of the BG pretreatment group were significantly lower than those in the 2 Gy group, and the enzyme activities of SOD and CAT were higher than those in the 2 Gy group. At 0.5 h and 24 h after irradiation, the repair rates of DNA damage in the BG pretreatment group were higher than those in the 2 Gy group. The results indicate that BG has a radioprotection effect on Eahy926 cells, and the mechanism may be attributed to BG’s effect on scavenge of ROS, enhancement of antioxidase activities, reduction of DNA damage and cell apoptosis, and promotion of the repair of DNA damage.

Key words: Saccharomyces cerevisiae-derived-beta-D-glucan, X-rays, Human umbilical vein endothelial cell (Eahy926), Radioprotection

CLC Number: 

  • TL71