Journal of Radiation Research and Radiation Proces ›› 2018, Vol. 36 ›› Issue (3): 30202-030202.

### Effect of 0.4 mT power frequency magnetic field on the migration of human amniotic epithelial cells

YANG Fan, LIU He, QI Hongxin, ZHANG Jie, XIA Ruohong, WANG Xianghui

1. Biophysics Laboratory, School of Physics and Material Science, East China Normal University, Shanghai 200241, China
• Received:2018-02-01 Revised:2018-03-18 Online:2018-06-20 Published:2018-06-21
• Supported by:

Supported by National Program on Key Basic Research Project (973 program) (2011CB503703)

Abstract:

To study the molecular mechanism of the effect of a 0.4 mT power frequency magnetic field (PFMF) on the mobility of human amniotic epithelial (FL) cells, immunofluorescence labeling and western blotting were used to detect the distribution, expression, and activity of two key regulatory proteins, FAK and Paxillin, which function in the focal adhesion signaling pathway. Subsequently, cell migration was evaluated to verify whether FAK participated in PFMF-activated cell migration. The results showed that compared to the sham group, the number of FAK and Paxillin foci in the MF group increased at cell borders, which implied that the total number of focal adhesion spots had increased. Moreover, the total content of Paxillin increased in the PFMF-exposed group. Although the levels of FAK were not significantly changed, Y397 phosphorylation of FAK was significantly upregulated. When the activity of the epidermal growth factor receptor (EGFR) was inhibited by PD153035 (PD), or the phosphorylation of Y397-FAK was inhibited by FAK Inhibitor 14 (FI), the above-mentioned effects were no longer seen. Cell migration experiments showed that PFMF promoted cell migration through upregulating the FAK phosphorylation level, because the effect was sensitive to the inhibition of Y397-FAK phosphorylation. All evidence indicated that the effect of PFMF on cell mobility was similar to that of the EGFR ligand, epidermal growth factor (EGF). Therefore, it can be concluded that 0.4 mT PFMF regulates the formation and distribution of focal adhesions by activating EGFR, which subsequently activates FAK and Paxillin, resulting in an increase in cell mobility via lamellipodial extension.

CLC Number:

• Q64